This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k2h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:57, 3 April 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k2h.jpg|left|200px]]
 
-
<!--
+
==Three-dimensional Solution Structure of apo-S100A1.==
-
The line below this paragraph, containing "STRUCTURE_1k2h", creates the "Structure Box" on the page.
+
<StructureSection load='1k2h' size='340' side='right'caption='[[1k2h]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1k2h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K2H FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2h OCA], [https://pdbe.org/1k2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k2h RCSB], [https://www.ebi.ac.uk/pdbsum/1k2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k2h ProSAT]</span></td></tr>
-
{{STRUCTURE_1k2h| PDB=1k2h | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/S10A1_RAT S10A1_RAT] Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k2h_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k2h ConSurf].
 +
<div style="clear:both"></div>
-
'''Three-dimensional Solution Structure of apo-S100A1.'''
+
==See Also==
-
 
+
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Baldiserri et al. (1999) J. Biomol. NMR 14, 87-88]. In this study, the three-dimensional structure of reduced apo-S100A1 was determined by NMR spectroscopy using a total of 2220 NOE distance constraints, 258 dihedral angle constraints, and 168 backbone hydrogen bond constraints derived from a series of 2D, 3D, and 4D NMR experiments. The final structure was found to be globular and compact with the four helices in each subunit aligning to form a unicornate-type four-helix bundle. Intermolecular NOE correlations were observed between residues in helices 1 and 4 from one subunit to residues in helices 1' and 4' of the other subunit, respectively, consistent with the antiparallel alignment of the two subunits to form a symmetric X-type four-helix bundle as found for other members of the S100 protein family. Because of the similarity of the S100A1 dimer interface to that found for S100B, it was possible to calculate a model of the S100A1/B heterodimer. This model is consistent with a number of NMR chemical shift changes observed when S100A1 is titrated into a sample of (15)N-labeled S100B. Helix 3 (and 3') of S100A1 was found to have an interhelical angle of -150 degrees with helix 4 (and 4') in the apo state. This crossing angle is quite different (&gt;50 degrees ) from that typically found in other EF-hand containing proteins such as apocalmodulin and apotroponin C but more similar to apo-S100B, which has an interhelical angle of -166 degrees. As with S100B, it is likely that the second EF-hand of apo-S100A1 reorients dramatically upon the addition of Ca(2+), which can explain the Ca(2+) dependence that S100A1 has for binding several of its biological targets.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1K2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2H OCA].
+
-
 
+
-
==Reference==
+
-
Three-dimensional solution structure of the calcium-signaling protein apo-S100A1 as determined by NMR., Rustandi RR, Baldisseri DM, Inman KG, Nizner P, Hamilton SM, Landar A, Landar A, Zimmer DB, Weber DJ, Biochemistry. 2002 Jan 22;41(3):788-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11790100 11790100]
+
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Single protein]]
+
[[Category: Baldisseri DM]]
-
[[Category: Baldisseri, D M.]]
+
[[Category: Hamilton SM]]
-
[[Category: Hamilton, S M.]]
+
[[Category: Inman KG]]
-
[[Category: Inman, K G.]]
+
[[Category: Landar A]]
-
[[Category: Landar, A.]]
+
[[Category: Nizner P]]
-
[[Category: Nizner, P.]]
+
[[Category: Rustandi RR]]
-
[[Category: Rustandi, R R.]]
+
[[Category: Weber DJ]]
-
[[Category: Weber, D J.]]
+
[[Category: Zimmer DB]]
-
[[Category: Zimmer, D B.]]
+
-
[[Category: Non-covalent homodimer]]
+
-
[[Category: X-type four-helix bundle]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:13:14 2008''
+

Current revision

Three-dimensional Solution Structure of apo-S100A1.

PDB ID 1k2h

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k2h"
Personal tools