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1k3f

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[[Image:1k3f.jpg|left|200px]]
 
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==Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice==
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The line below this paragraph, containing "STRUCTURE_1k3f", creates the "Structure Box" on the page.
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<StructureSection load='1k3f' size='340' side='right'caption='[[1k3f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k3f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K3F FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3f OCA], [https://pdbe.org/1k3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k3f RCSB], [https://www.ebi.ac.uk/pdbsum/1k3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3f ProSAT]</span></td></tr>
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{{STRUCTURE_1k3f| PDB=1k3f | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UDP_ECOLI UDP_ECOLI] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/1k3f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k3f ConSurf].
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<div style="clear:both"></div>
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'''Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice'''
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==See Also==
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*[[Uridine phosphorylase 3D structures|Uridine phosphorylase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments.
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==About this Structure==
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1K3F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3F OCA].
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==Reference==
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Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice., Morgunova EYu, Mikhailov AM, Popov AN, Blagova EV, Smirnova EA, Vainshtein BK, Mao C, Armstrong ShR, Ealick SE, Komissarov AA, et al., FEBS Lett. 1995 Jun 26;367(2):183-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7796917 7796917]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Uridine phosphorylase]]
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[[Category: Armstrong SR]]
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[[Category: Armstrong, S R.]]
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[[Category: Blagova EV]]
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[[Category: Blagova, E V.]]
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[[Category: Burlakova AA]]
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[[Category: Burlakova, A A.]]
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[[Category: Debabov VG]]
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[[Category: Debabov, V G.]]
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[[Category: Ealick SE]]
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[[Category: Ealick, S E.]]
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[[Category: Komissarov AA]]
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[[Category: Komissarov, A A.]]
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[[Category: Linkova EV]]
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[[Category: Linkova, E V.]]
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[[Category: Mao C]]
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[[Category: Mao, C.]]
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[[Category: Mikhailov AM]]
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[[Category: Mikhailov, A M.]]
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[[Category: Mironov AS]]
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[[Category: Mironov, A S.]]
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[[Category: Morgunova EYu]]
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[[Category: Morgunova, E Yu.]]
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[[Category: Popov AN]]
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[[Category: Popov, A N.]]
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[[Category: Smirnova EA]]
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[[Category: Smirnova, E A.]]
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[[Category: Vainshtein BK]]
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[[Category: Vainshtein, B K.]]
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[[Category: Hexamer]]
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[[Category: Nucleoside phosphorylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:15:09 2008''
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Current revision

Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice

PDB ID 1k3f

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