8cf7
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Dimethylated RSL-R5 in complex with cucurbit[7]uril, C121 sheet assembly== | |
| + | <StructureSection load='8cf7' size='340' side='right'caption='[[8cf7]], [[Resolution|resolution]] 1.14Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8cf7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CF7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.14Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=QQ7:CUCURBIT[7]URIL'>QQ7</scene>, <scene name='pdbligand=SNM:N,N-dimethyl-L-serine'>SNM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cf7 OCA], [https://pdbe.org/8cf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cf7 RCSB], [https://www.ebi.ac.uk/pdbsum/8cf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cf7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The donut-shaped cucurbit[n]urils (Qn) are a class of rigid macrocyclic receptor with protein recognition capabilities. Qn encapsulation of amino acid side chains can enable protein assembly. Recently, cucurbit[7]uril (Q7) has been applied as a molecular glue for organizing protein building blocks into crystalline architectures. Q7 co-crystallization with dimethylated Ralstonia solanacearum lectin (RSL*) has yielded novel crystalline architectures. Co-crystallization of RSL* and Q7 yields either cage- or sheet-like architectures which may be modulated via protein engineering. However, questions remain as to the factors dictating the formation of one architecture over another (cage versus sheet). Here, we make use of an engineered RSL*-Q7 system which co-crystallizes as the cage or sheet assembly with easily-distinguished crystal morphologies. Using this model system, we probe how the crystallization conditions dictate which crystalline architecture is adopted. Protein-ligand ratios and the sodium concentration were identified as key determinants for the growth of the cage versus sheet assemblies. | ||
| - | + | Cage versus sheet: Probing the Determinants of Protein - Cucurbit[7]uril Crystalline Architectures.,Ramberg KO, Crowley PB J Struct Biol. 2023 Jun;215(2):107969. doi: 10.1016/j.jsb.2023.107969. Epub 2023 , May 1. PMID:37137399<ref>PMID:37137399</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Crowley | + | <div class="pdbe-citations 8cf7" style="background-color:#fffaf0;"></div> |
| - | [[Category: Ramberg | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ralstonia solanacearum]] | ||
| + | [[Category: Crowley PB]] | ||
| + | [[Category: Ramberg K]] | ||
Current revision
Dimethylated RSL-R5 in complex with cucurbit[7]uril, C121 sheet assembly
| |||||||||||
