This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k6i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:44, 7 February 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k6i.gif|left|200px]]
 
-
<!--
+
==Crystal structure of Nmra, a negative transcriptional regulator (Trigonal form)==
-
The line below this paragraph, containing "STRUCTURE_1k6i", creates the "Structure Box" on the page.
+
<StructureSection load='1k6i' size='340' side='right'caption='[[1k6i]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1k6i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K6I FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-
{{STRUCTURE_1k6i| PDB=1k6i | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6i OCA], [https://pdbe.org/1k6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k6i RCSB], [https://www.ebi.ac.uk/pdbsum/1k6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k6i ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Crystal structure of Nmra, a negative transcriptional regulator (Trigonal form)'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/NMRA_EMENI NMRA_EMENI] May be a redox sensor protein. Has much higher affinity for NAD(P) than for NAD(P)H. Has similar affinity for NAD and NADP. Negative transcriptional regulator involved in the post-transcriptional modulation of the GATA-type transcription factor areA, forming part of a system controlling nitrogen metabolite repression (By similarity). Interferes with the interaction between areA and target DNA. Overexpression leads to areA inhibition.<ref>PMID:17854403</ref> <ref>PMID:12764138</ref> <ref>PMID:15537757</ref> <ref>PMID:18602114</ref>
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
NmrA is a negative transcriptional regulator involved in the post-translational modulation of the GATA-type transcription factor AreA, forming part of a system controlling nitrogen metabolite repression in various fungi. X-ray structures of two NmrA crystal forms, both to 1.8 A resolution, show NmrA consists of two domains, including a Rossmann fold. NmrA shows an unexpected similarity to the short-chain dehydrogenase/reductase (SDR) family, with the closest relationship to UDP-galactose 4-epimerase. We show that NAD binds to NmrA, a previously unreported nucleotide binding property for this protein. NmrA is unlikely to be an active dehydrogenase, however, as the conserved catalytic tyrosine in SDRs is absent in NmrA, and thus the nucleotide binding to NmrA could have a regulatory function. Our results suggest that other transcription factors possess the SDR fold with functions including RNA binding. The SDR fold appears to have been adapted for other roles including non-enzymatic control functions such as transcriptional regulation and is likely to be more widespread than previously recognized.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k6i_consurf.spt"</scriptWhenChecked>
-
1K6I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6I OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases., Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR, EMBO J. 2001 Dec 3;20(23):6619-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726498 11726498]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k6i ConSurf].
-
[[Category: Emericella nidulans]]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
== References ==
-
[[Category: Cocklin, S.]]
+
<references/>
-
[[Category: Dodds, A.]]
+
__TOC__
-
[[Category: Hawkins, A R.]]
+
</StructureSection>
-
[[Category: Lamb, H K.]]
+
[[Category: Aspergillus nidulans]]
-
[[Category: Leslie, K.]]
+
[[Category: Large Structures]]
-
[[Category: Nichols, C E.]]
+
[[Category: Cocklin S]]
-
[[Category: Ren, J.]]
+
[[Category: Dodds A]]
-
[[Category: Stammers, D K.]]
+
[[Category: Hawkins AR]]
-
[[Category: Rossmann fold transcriptional regulation short chain dehydrogenase reductase]]
+
[[Category: Lamb HK]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:21:57 2008''
+
[[Category: Leslie K]]
 +
[[Category: Nichols CE]]
 +
[[Category: Ren J]]
 +
[[Category: Stammers DK]]

Current revision

Crystal structure of Nmra, a negative transcriptional regulator (Trigonal form)

PDB ID 1k6i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k6i"
Personal tools