8in2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8in2 is ON HOLD Authors: Chiang, C.C., Hsu, C.H. Description: 4,5-DOPA-extradiol-dioxygenase from Beta vulgaris Category: Unreleased Structures...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==4,5-DOPA-extradiol-dioxygenase from Beta vulgaris== | |
| + | <StructureSection load='8in2' size='340' side='right'caption='[[8in2]], [[Resolution|resolution]] 3.08Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8in2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IN2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.08Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8in2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8in2 OCA], [https://pdbe.org/8in2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8in2 RCSB], [https://www.ebi.ac.uk/pdbsum/8in2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8in2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Betalains, a group of pigments widely distributed in various plants, are extensively applied in the food, beverage, and medicinal industries. The biosynthesis of betalains involves the enzymatic action of 4,5-DOPA-dioxygenase, which catalyzes the key ring-opening reaction of DOPA to produce betalamic acid, a crucial intermediate in the pathway. The crystal structure of a 4,5-DOPA-dioxygenase from Beta vulgaris (BvDOD) was determined in this study. The structural analysis revealed that BvDOD exhibited a structural fold similar to that of other members of the extradiol dioxygenase family. Moreover, the Fe-ligand residues His15, His53, and His229 indicated the enzyme's reliance on nonheme iron for catalyzing the ring-opening reaction. Molecular docking and mutational analysis identified two conserved residues, His119 and His175, in the active site essential for the catalytic reaction. In addition, Thr17, Asp254, and Tyr260 contributed to properly positioning the substrate in the active site. This study has provided structural insights into substrate recognition and catalytic mechanisms of BvDOD, which can be applied to develop enzymes for improved betalain production. | ||
| - | + | Structural Insights into 4,5-DOPA Extradiol Dioxygenase from Beta vulgaris: Unraveling the Key Step in Versatile Betalain Biosynthesis.,Chiang CC, Lu YJ, Liu JW, Lin SW, Chou CC, Lin CH, Chien IW, Hsu CH J Agric Food Chem. 2025 Mar 19;73(11):6785-6794. doi: 10.1021/acs.jafc.4c09501. , Epub 2025 Mar 7. PMID:40055856<ref>PMID:40055856</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Chiang | + | <div class="pdbe-citations 8in2" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hsu | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Beta vulgaris]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chiang CC]] | ||
| + | [[Category: Hsu CH]] | ||
Current revision
4,5-DOPA-extradiol-dioxygenase from Beta vulgaris
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