8b8v
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8b8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B8V FirstGlance]. <br> | <table><tr><td colspan='2'>[[8b8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B8V FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b8v OCA], [https://pdbe.org/8b8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b8v RCSB], [https://www.ebi.ac.uk/pdbsum/8b8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b8v ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b8v OCA], [https://pdbe.org/8b8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b8v RCSB], [https://www.ebi.ac.uk/pdbsum/8b8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b8v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/D6Q0Q6_9RHAB D6Q0Q6_9RHAB] | [https://www.uniprot.org/uniprot/D6Q0Q6_9RHAB D6Q0Q6_9RHAB] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | As for all non-segmented negative RNA viruses, rabies virus has its genome packaged in a linear assembly of nucleoprotein (N), named nucleocapsid. The formation of new nucleocapsids during virus replication in cells requires the production of soluble N protein in complex with its phosphoprotein (P) chaperone. In this study, we reconstituted a soluble heterodimeric complex between an armless N protein of rabies virus (RABV), lacking its N-terminal subdomain (N(NT-ARM)), and a peptide encompassing the N(0) chaperon module of the P protein. We showed that the chaperone module undergoes a disordered-order transition when it assembles with N(0) and measured an affinity in the low nanomolar range using a competition assay. We solved the crystal structure of the complex at a resolution of 2.3 A, unveiling the details of the conserved interfaces. MD simulations showed that both the chaperon module of P and RNA-mediated polymerization reduced the ability of the RNA binding cavity to open and close. Finally, by reconstituting a complex with full-length P protein, we demonstrated that each P dimer could independently chaperon two N(0) molecules. | ||
| - | + | ==See Also== | |
| - | + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of the Rabies virus RNA free nucleoprotein- phosphoprotein complex
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