1jfi

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(New page: 200px<br /> <applet load="1jfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jfi, resolution 2.62&Aring;" /> '''Crystal Structure o...)
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[[Image:1jfi.gif|left|200px]]<br />
 
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<applet load="1jfi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jfi, resolution 2.62&Aring;" />
 
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'''Crystal Structure of the NC2-TBP-DNA Ternary Complex'''<br />
 
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==Overview==
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==Crystal Structure of the NC2-TBP-DNA Ternary Complex==
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The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the, TATA box binding protein (TBP), and DNA has been determined at 2.6 A, resolution. The N termini of NC2 alpha and beta resemble histones H2A and, H2B, respectively, and form a heterodimer that binds to the bent DNA, double helix on the underside of the preformed TBP-DNA complex via, electrostatic interactions. NC2beta contributes to inhibition of, TATA-dependent transcription through interactions of its C-terminal alpha, helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block, recognition of the TBP-DNA complex by transcription factor IIB. Further, regulatory implications of the NC2 heterodimer structure are discussed.
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<StructureSection load='1jfi' size='340' side='right'caption='[[1jfi]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jfi]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The July 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TATA-Binding Protein'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_7 10.2210/rcsb_pdb/mom_2005_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JFI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jfi OCA], [https://pdbe.org/1jfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jfi RCSB], [https://www.ebi.ac.uk/pdbsum/1jfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jfi ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1jfi TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NC2A_HUMAN NC2A_HUMAN] The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.<ref>PMID:8670811</ref> <ref>PMID:8608938</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jfi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jfi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.
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==Disease==
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Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.,Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK Cell. 2001 Jul 13;106(1):71-81. PMID:11461703<ref>PMID:11461703</ref>
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Known diseases associated with this structure: Glioblastoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606417 606417]], Huntington disease-like-4 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Parkinson disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Spinocerebellar ataxia 17 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1JFI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1JFI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JFI OCA].
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</div>
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<div class="pdbe-citations 1jfi" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex., Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK, Cell. 2001 Jul 13;106(1):71-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11461703 11461703]
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*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: TATA-Binding Protein]]
[[Category: TATA-Binding Protein]]
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[[Category: Burley, S.K.]]
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[[Category: Burley SK]]
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[[Category: Chen, H.]]
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[[Category: Chen H]]
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[[Category: Kamada, K.]]
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[[Category: Kamada K]]
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[[Category: Malik, S.]]
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[[Category: Malik S]]
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[[Category: Meisterernst, M.]]
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[[Category: Meisterernst M]]
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[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
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[[Category: Roeder RG]]
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[[Category: Roeder, R.G.]]
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[[Category: Shu F]]
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[[Category: Shu, F.]]
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[[Category: Stelzer G]]
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[[Category: Stelzer, G.]]
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[[Category: h2a/h2b]]
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[[Category: histone]]
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[[Category: nc2]]
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[[Category: negative cofactor]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tata-dna]]
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[[Category: tbp]]
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[[Category: transcription initiation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:40:24 2007''
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Current revision

Crystal Structure of the NC2-TBP-DNA Ternary Complex

PDB ID 1jfi

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