8bf8
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8bf8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BF8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[8bf8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BF8 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bf8 OCA], [https://pdbe.org/8bf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bf8 RCSB], [https://www.ebi.ac.uk/pdbsum/8bf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bf8 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bf8 OCA], [https://pdbe.org/8bf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bf8 RCSB], [https://www.ebi.ac.uk/pdbsum/8bf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bf8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/DRA2B_DEIRA DRA2B_DEIRA] | [https://www.uniprot.org/uniprot/DRA2B_DEIRA DRA2B_DEIRA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells(1,2). Bioinformatic analysis identified TnpB proteins as the likely predecessors of Cas12 nucleases(3-5), which along with Cas9 are widely used for targeted genome manipulation. Whereas Cas12 family nucleases are well characterized both biochemically and structurally(6), the molecular mechanism of TnpB remains unknown. Here we present the cryogenic-electron microscopy structures of the Deinococcus radiodurans TnpB-reRNA (right-end transposon element-derived RNA) complex in DNA-bound and -free forms. The structures reveal the basic architecture of TnpB nuclease and the molecular mechanism for DNA target recognition and cleavage that is supported by biochemical experiments. Collectively, these results demonstrate that TnpB represents the minimal structural and functional core of the Cas12 protein family and provide a framework for developing TnpB-based genome editing tools. | ||
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| + | TnpB structure reveals minimal functional core of Cas12 nuclease family.,Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V Nature. 2023 Apr;616(7956):384-389. doi: 10.1038/s41586-023-05826-x. Epub 2023 , Apr 5. PMID:37020015<ref>PMID:37020015</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8bf8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
ISDra2 TnpB in complex with reRNA
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