4wyv

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Human Translin in Open Conformation

Structural highlights

4wyv is a 8 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 4jhf. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSN_HUMAN DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.^[1] ^[2] Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.^[3] ^[4]

Publication Abstract from PubMed

The single-stranded DNA (ssDNA)/RNA binding protein translin was suggested to be involved in chromosomal translocations, telomere metabolism, and mRNA transport and translation. Oligonucleotide binding surfaces map within a closed cavity of translin octameric barrels, raising the question as to how DNA/RNA gain access to this inner cavity, particularly given that, to date, none of the barrel structures reported hint to an entryway. Here, we argue against a mechanism by which translin octamers may "dissociate and reassemble" upon RNA binding and report a novel "open"-barrel structure of human translin revealing a feasible DNA/RNA entryway into the cavity. Additionally, we report that translin not only is confined to binding of ssDNA oligonucleotides, or single-stranded extensions of double-stranded DNA (dsDNA), but also can bind single-stranded sequences internally embedded in dsDNA molecules.

A Novel Open-Barrel Structure of Octameric Translin Reveals a Potential RNA Entryway.,Eliahoo E, Marx A, Manor H, Alian A J Mol Biol. 2014 Nov 26. pii: S0022-2836(14)00609-3. doi:, 10.1016/j.jmb.2014.11.013. PMID:25433126^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K. The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. J Biol Chem. 1997 Apr 25;272(17):11402-7. PMID:9111049
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032
↑ Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K. The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. J Biol Chem. 1997 Apr 25;272(17):11402-7. PMID:9111049
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032
↑ Eliahoo E, Marx A, Manor H, Alian A. A Novel Open-Barrel Structure of Octameric Translin Reveals a Potential RNA Entryway. J Mol Biol. 2014 Nov 26. pii: S0022-2836(14)00609-3. doi:, 10.1016/j.jmb.2014.11.013. PMID:25433126 doi:http://dx.doi.org/10.1016/j.jmb.2014.11.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wyv"

Categories: Homo sapiens | Large Structures | Alian A | Dvir H | Eliahoo E

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4wyv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4jhf 4jhf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYV FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyv OCA], [https://pdbe.org/4wyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyv RCSB], [https://www.ebi.ac.uk/pdbsum/4wyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyv ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyv OCA], [https://pdbe.org/4wyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyv RCSB], [https://www.ebi.ac.uk/pdbsum/4wyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyv ProSAT]</span></td></tr>
 </table>
 == Function ==

4wyv

From Proteopedia

Current revision

Crystal Structure of Human Translin in Open Conformation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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