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1jow

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(New page: 200px<br /> <applet load="1jow" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jow, resolution 3.10&Aring;" /> '''Crystal structure o...)
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[[Image:1jow.gif|left|200px]]<br />
 
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<applet load="1jow" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jow, resolution 3.10&Aring;" />
 
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'''Crystal structure of a complex of human CDK6 and a viral cyclin'''<br />
 
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==Overview==
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==Crystal structure of a complex of human CDK6 and a viral cyclin==
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Cyclin from herpesvirus saimiri (Vcyclin) preferentially forms complexes, with cyclin-dependent kinase 6 (CDK6) from primate host cells. These, complexes show higher kinase activity than host cell CDKs in complex with, cellular cyclins and are resistant to cyclin-dependent inhibitory proteins, (CDKIs). The crystal structure of human CDK6--Vcyclin in an active state, was determined to 3.1 A resolution to better understand the structural, basis of CDK6 activation by viral cyclins. The unphosphorylated CDK6 in, complex with Vcyclin has many features characteristic of, cyclinA-activated, phosphorylated CDK2. There are, however, differences in, the conformation at the tip of the T-loop and its interactions with, Vcyclin. Residues in the N-terminal extension of Vcyclin wrap around the, tip of the CDK6 T-loop and form a short beta-sheet with the T-loop, backbone. These interactions lead to a 20% larger buried surface in the, CDK6--Vcyclin interface than in the CDK2--cyclinA complex and are probably, largely responsible for the specificity of Vcyclin for CDK6 and resistance, of the complex to inhibition by INK-type CDKIs.
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<StructureSection load='1jow' size='340' side='right'caption='[[1jow]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jow]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saimiriine_gammaherpesvirus_2 Saimiriine gammaherpesvirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JOW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jow OCA], [https://pdbe.org/1jow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jow RCSB], [https://www.ebi.ac.uk/pdbsum/1jow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jow ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CGH2_SHV21 CGH2_SHV21] May be highly relevant to the process of cellular transformation and rapid T-cell proliferation effected by HVS during latent infections of T-cells in susceptible hosts.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/1jow_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jow ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JOW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saimiriine_herpesvirus_3 Saimiriine herpesvirus 3]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JOW OCA].
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*[[Cyclin 3D structures|Cyclin 3D structures]]
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*[[Cyclin-dependent kinase 3D structures|Cyclin-dependent kinase 3D structures]]
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==Reference==
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__TOC__
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Structural basis for CDK6 activation by a virus-encoded cyclin., Schulze-Gahmen U, Kim SH, Nat Struct Biol. 2002 Mar;9(3):177-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11828325 11828325]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Saimiriine herpesvirus 3]]
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[[Category: Saimiriine gammaherpesvirus 2]]
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[[Category: Kim, S.H.]]
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[[Category: Kim SH]]
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[[Category: Schulze-Gahmen, U.]]
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[[Category: Schulze-Gahmen U]]
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[[Category: cdk-cyclin complex]]
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[[Category: cyclin fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:43:05 2007''
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Current revision

Crystal structure of a complex of human CDK6 and a viral cyclin

PDB ID 1jow

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