1kju

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:25, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1kju.gif|left|200px]]
 
-
<!--
+
==Ca2+-ATPase in the E2 State==
-
The line below this paragraph, containing "STRUCTURE_1kju", creates the "Structure Box" on the page.
+
<SX load='1kju' size='340' side='right' viewer='molstar' caption='[[1kju]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1kju]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KJU FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kju OCA], [https://pdbe.org/1kju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kju RCSB], [https://www.ebi.ac.uk/pdbsum/1kju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kju ProSAT]</span></td></tr>
-
{{STRUCTURE_1kju| PDB=1kju | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/1kju_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kju ConSurf].
 +
<div style="clear:both"></div>
-
'''Ca2+-ATPase in the E2 State'''
+
==See Also==
-
 
+
*[[ATPase 3D structures|ATPase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</SX>
-
Ca(2+)-ATPase is responsible for active transport of calcium ions across the sarcoplasmic reticulum membrane. This coupling involves an ordered sequence of reversible reactions occurring alternately at the ATP site within the cytoplasmic domains, or at the calcium transport sites within the transmembrane domain. These two sites are separated by a large distance and conformational changes have long been postulated to play an important role in their coordination. To characterize the nature of these conformational changes, we have built atomic models for two reaction intermediates and postulated the mechanisms governing the large structural changes. One model is based on fitting the X-ray crystallographic structure of Ca(2+)-ATPase in the E1 state to a new 6 A structure by cryoelectron microscopy in the E2 state. This fit indicates that calcium binding induces enormous movements of all three cytoplasmic domains as well as significant changes in several transmembrane helices. We found that fluorescein isothiocyanate displaced a decavanadate molecule normally located at the intersection of the three cytoplasmic domains, but did not affect their juxtaposition; this result indicates that our model likely reflects a native E2 conformation and not an artifact of decavanadate binding. To explain the dramatic structural effect of calcium binding, we propose that M4 and M5 transmembrane helices are responsive to calcium binding and directly induce rotation of the phosphorylation domain. Furthermore, we hypothesize that both the nucleotide-binding and beta-sheet domains are highly mobile and driven by Brownian motion to elicit phosphoenzyme formation and calcium transport, respectively. If so, the reaction cycle of Ca(2+)-ATPase would have elements of a Brownian ratchet, where the chemical reactions of ATP hydrolysis are used to direct the random thermal oscillations of an innately flexible molecule.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1KJU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJU OCA].
+
-
 
+
-
==Reference==
+
-
A structural model for the catalytic cycle of Ca(2+)-ATPase., Xu C, Rice WJ, He W, Stokes DL, J Mol Biol. 2002 Feb 8;316(1):201-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11829513 11829513]
+
-
[[Category: Calcium-transporting ATPase]]
+
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
-
[[Category: Single protein]]
+
[[Category: He W]]
-
[[Category: He, W.]]
+
[[Category: Rice WJ]]
-
[[Category: Rice, W J.]]
+
[[Category: Stokes DL]]
-
[[Category: Stokes, D L.]]
+
[[Category: Xu C]]
-
[[Category: Xu, C.]]
+
-
[[Category: Active transport]]
+
-
[[Category: Calcium]]
+
-
[[Category: Cryo-em]]
+
-
[[Category: E2]]
+
-
[[Category: Ion pump]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: P-type atpase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:49:44 2008''
+

Current revision

Ca2+-ATPase in the E2 State

1kju, resolution 6.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kju"
Personal tools