1kjz

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[[Image:1kjz.gif|left|200px]]
 
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==Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant==
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The line below this paragraph, containing "STRUCTURE_1kjz", creates the "Structure Box" on the page.
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<StructureSection load='1kjz' size='340' side='right'caption='[[1kjz]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kjz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KJZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1kjz| PDB=1kjz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjz OCA], [https://pdbe.org/1kjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kjz RCSB], [https://www.ebi.ac.uk/pdbsum/1kjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjz ProSAT]</span></td></tr>
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</table>
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'''Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant'''
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== Function ==
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[https://www.uniprot.org/uniprot/IF2G_PYRAB IF2G_PYRAB] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/1kjz_consurf.spt"</scriptWhenChecked>
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1KJZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJZ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The large subunit of initiation factor aIF2 is a close structural homologue of elongation factors., Schmitt E, Blanquet S, Mechulam Y, EMBO J. 2002 Apr 2;21(7):1821-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11927566 11927566]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kjz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus abyssi]]
[[Category: Pyrococcus abyssi]]
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[[Category: Single protein]]
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[[Category: Blanquet S]]
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[[Category: Blanquet, S.]]
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[[Category: Mechulam Y]]
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[[Category: Mechulam, Y.]]
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[[Category: Schmitt E]]
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[[Category: Schmitt, E.]]
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[[Category: Initiation of translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:50:03 2008''
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Current revision

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant

PDB ID 1kjz

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