8opr
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8opr is ON HOLD Authors: Sogues, A., Remaut, H. Description: Structure of the EA1 surface layer of Bacillus anthracis [[Category: Unreleased Struct...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the EA1 surface layer of Bacillus anthracis== | |
| + | <StructureSection load='8opr' size='340' side='right'caption='[[8opr]], [[Resolution|resolution]] 1.81Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8opr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OPR FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.811Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8opr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8opr OCA], [https://pdbe.org/8opr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8opr RCSB], [https://www.ebi.ac.uk/pdbsum/8opr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8opr ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SLAP2_BACAN SLAP2_BACAN] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Gram-positive spore-forming bacterium Bacillus anthracis is the causative agent of anthrax, a deadly disease mostly affecting wildlife and livestock, as well as representing a bioterrorism threat. Its cell surface is covered by the mutually exclusive S-layers Sap and EA1, found in early and late growth phases, respectively. Here we report the nanobody-based structural characterization of EA1 and its native lattice contacts. The EA1 assembly domain consists of 6 immunoglobulin-like domains, where three calcium-binding sites structure interdomain contacts that allow monomers to adopt their assembly-competent conformation. Nanobody-induced depolymerization of EA1 S-layers results in surface defects, membrane blebbing and cell lysis under hypotonic conditions, indicating that S-layers provide additional mechanical stability to the cell wall. Taken together, we report a complete model of the EA1 S-layer and present a set of nanobodies that may have therapeutic potential against Bacillus anthracis. | ||
| - | + | Structure and function of the EA1 surface layer of Bacillus anthracis.,Sogues A, Fioravanti A, Jonckheere W, Pardon E, Steyaert J, Remaut H Nat Commun. 2023 Nov 3;14(1):7051. doi: 10.1038/s41467-023-42826-x. PMID:37923757<ref>PMID:37923757</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Remaut | + | <div class="pdbe-citations 8opr" style="background-color:#fffaf0;"></div> |
| - | [[Category: Sogues | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus anthracis]] | ||
| + | [[Category: Lama glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Remaut H]] | ||
| + | [[Category: Sogues A]] | ||
Current revision
Structure of the EA1 surface layer of Bacillus anthracis
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