8osi
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8osi is ON HOLD Authors: Varfolomeeva, L.A., Boyko, K.M., Subach, O.M., Subach, F.V. Description: Genetically encoded green ratiometric calcium ind...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state== | |
| + | <StructureSection load='8osi' size='340' side='right'caption='[[8osi]], [[Resolution|resolution]] 2.42Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8osi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aureobasidium_namibiae_CBS_147.97 Aureobasidium namibiae CBS 147.97] and [https://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OSI FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8osi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8osi OCA], [https://pdbe.org/8osi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8osi RCSB], [https://www.ebi.ac.uk/pdbsum/8osi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8osi ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1S4NYF2_BRALA A0A1S4NYF2_BRALA] [https://www.uniprot.org/uniprot/A0A254U4M1_ASPNG A0A254U4M1_ASPNG] [https://www.uniprot.org/uniprot/A0A100IBH9_ASPNG A0A100IBH9_ASPNG] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator. | ||
| - | + | FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.,Subach OM, Varfolomeeva L, Vlaskina AV, Agapova YK, Nikolaeva AY, Piatkevich KD, Patrushev MV, Boyko KM, Subach FV Biochem Biophys Res Commun. 2023 Jul 12;665:169-177. doi: , 10.1016/j.bbrc.2023.04.108. Epub 2023 Apr 28. PMID:37163937<ref>PMID:37163937</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8osi" style="background-color:#fffaf0;"></div> |
| - | [[Category: Subach | + | == References == |
| - | [[Category: Subach | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Aureobasidium namibiae CBS 147 97]] | ||
| + | [[Category: Branchiostoma lanceolatum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Boyko KM]] | ||
| + | [[Category: Nikolaeva AY]] | ||
| + | [[Category: Subach FV]] | ||
| + | [[Category: Subach OM]] | ||
| + | [[Category: Varfolomeeva LA]] | ||
Current revision
Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state
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