7y7v
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7y7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y7V FirstGlance]. <br> | <table><tr><td colspan='2'>[[7y7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y7V FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y7v OCA], [https://pdbe.org/7y7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y7v RCSB], [https://www.ebi.ac.uk/pdbsum/7y7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y7v ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y7v OCA], [https://pdbe.org/7y7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y7v RCSB], [https://www.ebi.ac.uk/pdbsum/7y7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y7v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Disease == | == Disease == | ||
| - | [https://www.uniprot.org/uniprot/SC6A1_HUMAN SC6A1_HUMAN] Myoclonic-astatic epilepsy. The disease is caused by variants affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/SC6A1_HUMAN SC6A1_HUMAN] Myoclonic-astatic epilepsy;Autosomal dominant non-syndromic intellectual disability. The disease is caused by variants affecting the gene represented in this entry. |
== Function == | == Function == | ||
| - | [https://www.uniprot.org/uniprot/SC6A1_HUMAN SC6A1_HUMAN] | + | [https://www.uniprot.org/uniprot/SC6A1_HUMAN SC6A1_HUMAN] Mediates transport of gamma-aminobutyric acid (GABA) together with sodium and chloride and is responsible for the reuptake of GABA from the synapse (PubMed:30132828). The translocation of GABA, however, may also occur in the reverse direction leading to the release of GABA (By similarity). The direction and magnitude of GABA transport is a consequence of the prevailing thermodynamic conditions, determined by membrane potential and the intracellular and extracellular concentrations of Na(+), Cl(-) and GABA (By similarity). Can also mediate sodium- and chloride-dependent transport of hypotaurine but to a much lower extent as compared to GABA (By similarity).[UniProtKB:P23978][UniProtKB:P31648]<ref>PMID:30132828</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | gamma-Aminobutyric acid (GABA), an important inhibitory neurotransmitter in the central nervous system, is recycled through specific GABA transporters (GATs). GAT1, which is mainly expressed in the presynaptic terminals of axons, is a potential drug target of neurological disorders due to its essential role in GABA transport. Here we report four cryogenic electron microscopy structures of human GAT1, at resolutions of 2.2-3.2 A. GAT1 in substrate-free form or in complex with the antiepileptic drug tiagabine exhibits an inward-open conformation. In the presence of GABA or nipecotic acid, inward-occluded structures are captured. The GABA-bound structure reveals an interaction network bridged by hydrogen bonds and ion coordination for GABA recognition. The substrate-free structure unwinds the last helical turn of transmembrane helix TM1a to release sodium ions and substrate. Complemented by structure-guided biochemical analyses, our studies reveal detailed mechanism of GABA recognition and transport, and elucidate mode of action of the inhibitors, nipecotic acid and tiagabine. | ||
| + | |||
| + | Molecular basis for substrate recognition and transport of human GABA transporter GAT1.,Zhu A, Huang J, Kong F, Tan J, Lei J, Yuan Y, Yan C Nat Struct Mol Biol. 2023 Jul;30(7):1012-1022. doi: 10.1038/s41594-023-00983-z. , Epub 2023 Jul 3. PMID:37400655<ref>PMID:37400655</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7y7v" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Cryo-EM structure of human apo GABA transporter GAT1 in an inward-open state
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Categories: Homo sapiens | Large Structures | Huang J | Kong F | Lei J | Tan J | Yan C | Yuan Y | Zhu A
