8j7m
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==ion channel== | |
| + | <StructureSection load='8j7m' size='340' side='right'caption='[[8j7m]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8j7m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J7M FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j7m OCA], [https://pdbe.org/8j7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j7m RCSB], [https://www.ebi.ac.uk/pdbsum/8j7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j7m ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/R1FVI4_EMIHU R1FVI4_EMIHU] [https://www.uniprot.org/uniprot/R1EKX3_EMIHU R1EKX3_EMIHU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Voltage-gated sodium (Na(V)) channels mediate a plethora of electrical activities. Na(V) channels govern cellular excitability in response to depolarizing stimuli. Inactivation is an intrinsic property of Na(V) channels that regulates cellular excitability by controlling the channel availability. The fast inactivation, mediated by the Ile-Phe-Met (IFM) motif and the N-terminal helix (N-helix), has been well-characterized. However, the molecular mechanism underlying Na(V) channel slow inactivation remains elusive. Here, we demonstrate that the removal of the N-helix of Na(V)Eh (Na(V)Eh(DeltaN)) results in a slow-inactivated channel, and present cryo-EM structure of Na(V)Eh(DeltaN) in a potential slow-inactivated state. The structure features a closed activation gate and a dilated selectivity filter (SF), indicating that the upper SF and the inner gate could serve as a gate for slow inactivation. In comparison to the Na(V)Eh structure, Na(V)Eh(DeltaN) undergoes marked conformational shifts on the intracellular side. Together, our results provide important mechanistic insights into Na(V) channel slow inactivation. | ||
| - | + | Structural mechanism of voltage-gated sodium channel slow inactivation.,Chen H, Xia Z, Dong J, Huang B, Zhang J, Zhou F, Yan R, Shi Y, Gong J, Jiang J, Huang Z, Jiang D Nat Commun. 2024 May 1;15(1):3691. doi: 10.1038/s41467-024-48125-3. PMID:38693179<ref>PMID:38693179</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Chen | + | <div class="pdbe-citations 8j7m" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aequorea victoria]] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen HW]] | ||
| + | [[Category: Jiang D]] | ||
Current revision
ion channel
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