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1jxs

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(New page: 200px<br /> <applet load="1jxs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jxs" /> '''Solution Structure of the DNA-Binding Domai...)
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[[Image:1jxs.gif|left|200px]]<br />
 
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<applet load="1jxs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jxs" />
 
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'''Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor'''<br />
 
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==Overview==
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==Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor==
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Interleukin enhancer binding factor (ILF) binds to the interleukin-2, (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D, structure of the DNA-binding domain of ILF was determined by, multidimensional NMR spectroscopy. NMR structure analysis revealed that, the DNA-binding domain of ILF is a new member of the winged helix/forkhead, family, and that its wing 2 contains an extra alpha-helix. This is the, first study to report the presence of a C-terminal alpha-helix in place of, a typical wing 2 in a member of this family. This structural difference, may be responsible for the different DNA-binding specificity of ILF, compared to other winged helix/forkhead proteins. Our deletion studies of, the fragments of ILF also suggest that the C-terminal region plays a, regulatory role in DNA binding.
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<StructureSection load='1jxs' size='340' side='right'caption='[[1jxs]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jxs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JXS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jxs OCA], [https://pdbe.org/1jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jxs RCSB], [https://www.ebi.ac.uk/pdbsum/1jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jxs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FOXK2_HUMAN FOXK2_HUMAN] Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.<ref>PMID:1909027</ref> <ref>PMID:1339390</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jx/1jxs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jxs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.
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==About this Structure==
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Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a).,Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ Proteins. 2002 Dec 1;49(4):543-53. PMID:12402362<ref>PMID:12402362</ref>
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1JXS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JXS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)., Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ, Proteins. 2002 Dec 1;49(4):543-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12402362 12402362]
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</div>
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<div class="pdbe-citations 1jxs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, S.H.]]
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[[Category: Chen SH]]
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[[Category: Chen, S.W.]]
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[[Category: Chen SW]]
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[[Category: Chuang, W.J.]]
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[[Category: Chuang WJ]]
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[[Category: Hsieh, Y.H.]]
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[[Category: Hsieh YH]]
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[[Category: Jeng, W.Y.]]
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[[Category: Jeng WY]]
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[[Category: Li, C.]]
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[[Category: Li C]]
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[[Category: Liu, P.P.]]
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[[Category: Liu PP]]
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[[Category: dna-binding domain]]
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[[Category: winged helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:45:35 2007''
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Current revision

Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor

PDB ID 1jxs

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