8ste

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8ste]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8STE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8STE FirstGlance]. <br>
<table><tr><td colspan='2'>[[8ste]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8STE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8STE FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUN:5-(AMINOSULFONYL)-4-CHLORO-2-[(2-FURYLMETHYL)AMINO]BENZOIC+ACID'>FUN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUN:5-(AMINOSULFONYL)-4-CHLORO-2-[(2-FURYLMETHYL)AMINO]BENZOIC+ACID'>FUN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ste FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ste OCA], [https://pdbe.org/8ste PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ste RCSB], [https://www.ebi.ac.uk/pdbsum/8ste PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ste ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ste FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ste OCA], [https://pdbe.org/8ste PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ste RCSB], [https://www.ebi.ac.uk/pdbsum/8ste PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ste ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[https://www.uniprot.org/uniprot/A0A8C9GPS0_9PRIM A0A8C9GPS0_9PRIM]
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[https://www.uniprot.org/uniprot/S12A2_HUMAN S12A2_HUMAN] Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Na-K-2Cl cotransporter-1 (NKCC1) is an electroneutral Na(+)-dependent transporter responsible for simultaneously translocating Na(+), K(+), and Cl(-) ions into cells. In human tissue, NKCC1 plays a critical role in regulating cytoplasmic volume, fluid intake, chloride homeostasis, and cell polarity. Here, we report four structures of human NKCC1 (hNKCC1), both in the absence and presence of loop diuretic (bumetanide or furosemide), using single-particle cryo-electron microscopy. These structures allow us to directly observe various novel conformations of the hNKCC1 dimer. They also reveal two drug-binding sites located at the transmembrane and cytosolic carboxyl-terminal domains, respectively. Together, our findings enable us to delineate an inhibition mechanism that involves a coupled movement between the cytosolic and transmembrane domains of hNKCC1.
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Inhibition mechanism of NKCC1 involves the carboxyl terminus and long-range conformational coupling.,Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW Sci Adv. 2022 Oct 28;8(43):eabq0952. doi: 10.1126/sciadv.abq0952. Epub 2022 Oct , 28. PMID:36306358<ref>PMID:36306358</ref>
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==See Also==
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*[[Solute carrier family 12 3D structures|Solute carrier family 12 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 8ste" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>

Current revision

Cryo-EM structure of NKCC1 Fu_CTD

PDB ID 8ste

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