1k86

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(New page: 200px<br /> <applet load="1k86" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k86, resolution 2.6&Aring;" /> '''Crystal structure of...)
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[[Image:1k86.gif|left|200px]]<br />
 
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<applet load="1k86" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k86, resolution 2.6&Aring;" />
 
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'''Crystal structure of caspase-7'''<br />
 
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==Overview==
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==Crystal structure of caspase-7==
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Apoptosis is primarily executed by active caspases, which are derived from, the inactive procaspase zymogens through proteolytic cleavage. Here we, report the crystal structures of a caspase zymogen, procaspase-7, and an, active caspase-7 without any bound inhibitors. Compared to the, inhibitor-bound caspase-7, procaspase-7 zymogen exhibits significant, structural differences surrounding the catalytic cleft, which precludes, the formation of a productive conformation. Proteolytic cleavage between, the large and small subunits allows rearrangement of essential loops in, the active site, priming active caspase-7 for inhibitor/substrate binding., Strikingly, binding by inhibitors causes a 180 degrees flipping of the N, terminus in the small subunit, which interacts with and stabilizes the, catalytic cleft. These analyses reveal the structural mechanisms of, caspase activation and demonstrate that the inhibitor/substrate binding is, a process of induced fit.
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<StructureSection load='1k86' size='340' side='right'caption='[[1k86]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k86]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K86 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k86 OCA], [https://pdbe.org/1k86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k86 RCSB], [https://www.ebi.ac.uk/pdbsum/1k86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k86 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CASP7_HUMAN CASP7_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k86_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k86 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K86 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA].
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*[[Caspase 3D structures|Caspase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11701129 11701129]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alnemri, E.S.]]
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[[Category: Alnemri ES]]
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[[Category: Chai, J.]]
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[[Category: Chai J]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: Shiozaki, E.]]
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[[Category: Shiozaki E]]
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[[Category: Srinivasa, S.M.]]
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[[Category: Srinivasa SM]]
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[[Category: Wu, Q.]]
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[[Category: Wu Q]]
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[[Category: activation]]
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[[Category: apoptosis]]
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[[Category: caspase]]
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[[Category: zymogen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:48:10 2007''
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Current revision

Crystal structure of caspase-7

PDB ID 1k86

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