1k88

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(New page: 200px<br /> <applet load="1k88" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k88, resolution 2.7&Aring;" /> '''Crystal structure of...)
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[[Image:1k88.gif|left|200px]]<br />
 
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<applet load="1k88" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k88, resolution 2.7&Aring;" />
 
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'''Crystal structure of procaspase-7'''<br />
 
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==Overview==
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==Crystal structure of procaspase-7==
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Apoptosis is primarily executed by active caspases, which are derived from, the inactive procaspase zymogens through proteolytic cleavage. Here we, report the crystal structures of a caspase zymogen, procaspase-7, and an, active caspase-7 without any bound inhibitors. Compared to the, inhibitor-bound caspase-7, procaspase-7 zymogen exhibits significant, structural differences surrounding the catalytic cleft, which precludes, the formation of a productive conformation. Proteolytic cleavage between, the large and small subunits allows rearrangement of essential loops in, the active site, priming active caspase-7 for inhibitor/substrate binding., Strikingly, binding by inhibitors causes a 180 degrees flipping of the N, terminus in the small subunit, which interacts with and stabilizes the, catalytic cleft. These analyses reveal the structural mechanisms of, caspase activation and demonstrate that the inhibitor/substrate binding is, a process of induced fit.
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<StructureSection load='1k88' size='340' side='right'caption='[[1k88]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k88]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The August 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Caspases'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_8 10.2210/rcsb_pdb/mom_2004_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K88 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k88 OCA], [https://pdbe.org/1k88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k88 RCSB], [https://www.ebi.ac.uk/pdbsum/1k88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k88 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CASP7_HUMAN CASP7_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k88_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k88 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K88 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1K88 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb56_1.html Caspases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K88 OCA].
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*[[Caspase 3D structures|Caspase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11701129 11701129]
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[[Category: Caspases]]
[[Category: Caspases]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alnemri, E.S.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Chai, J.]]
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[[Category: Alnemri ES]]
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[[Category: Shi, Y.]]
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[[Category: Chai J]]
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[[Category: Shiozaki, E.]]
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[[Category: Shi Y]]
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[[Category: Srinivasa, S.M.]]
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[[Category: Shiozaki E]]
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[[Category: Wu, Q.]]
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[[Category: Srinivasa SM]]
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[[Category: apoptosis]]
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[[Category: Wu Q]]
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[[Category: procaspase activation]]
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[[Category: protease]]
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[[Category: substrate binding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:48:13 2007''
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Current revision

Crystal structure of procaspase-7

PDB ID 1k88

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