1kcg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1kcg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kcg, resolution 2.6&Aring;" /> '''NKG2D in complex wit...)
Current revision (09:38, 25 December 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1kcg.gif|left|200px]]<br />
 
-
<applet load="1kcg" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1kcg, resolution 2.6&Aring;" />
 
-
'''NKG2D in complex with ULBP3'''<br />
 
-
==Overview==
+
==NKG2D in complex with ULBP3==
-
NKG2D is known to trigger the natural killer (NK) cell lysis of various, tumor and virally infected cells. In the NKG2D/ULBP3 complex, the, structure of ULBP3 resembles the alpha1 and alpha2 domains of classical, MHC molecules without a bound peptide. The lack of alpha3 and beta2m, domains is compensated by replacing two hydrophobic patches at the, underside of the class I MHC-like beta sheet floor with a group of, hydrophilic and charged residues in ULBP3. NKG2D binds diagonally across, the ULBP3 alpha helices, creating a complementary interface, an, asymmetrical subunit orientation, and local conformational adjustments in, the receptor. The interface is stabilized primarily by hydrogen bonds and, hydrophobic interactions. Unlike the KIR receptors that recognize a, conserved HLA region by a lock-and-key mechanism, NKG2D recognizes diverse, ligands by an induced-fit mechanism.
+
<StructureSection load='1kcg' size='340' side='right'caption='[[1kcg]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1kcg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KCG FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CGL:CYSTINE-GLUTATHIONE'>CGL</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcg OCA], [https://pdbe.org/1kcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kcg RCSB], [https://www.ebi.ac.uk/pdbsum/1kcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kcg ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NKG2D_HUMAN NKG2D_HUMAN] Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/1kcg_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kcg ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
NKG2D is known to trigger the natural killer (NK) cell lysis of various tumor and virally infected cells. In the NKG2D/ULBP3 complex, the structure of ULBP3 resembles the alpha1 and alpha2 domains of classical MHC molecules without a bound peptide. The lack of alpha3 and beta2m domains is compensated by replacing two hydrophobic patches at the underside of the class I MHC-like beta sheet floor with a group of hydrophilic and charged residues in ULBP3. NKG2D binds diagonally across the ULBP3 alpha helices, creating a complementary interface, an asymmetrical subunit orientation, and local conformational adjustments in the receptor. The interface is stabilized primarily by hydrogen bonds and hydrophobic interactions. Unlike the KIR receptors that recognize a conserved HLA region by a lock-and-key mechanism, NKG2D recognizes diverse ligands by an induced-fit mechanism.
-
==About this Structure==
+
Conformational plasticity revealed by the cocrystal structure of NKG2D and its class I MHC-like ligand ULBP3.,Radaev S, Rostro B, Brooks AG, Colonna M, Sun PD Immunity. 2001 Dec;15(6):1039-49. PMID:11754823<ref>PMID:11754823</ref>
-
1KCG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GSH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KCG OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Conformational plasticity revealed by the cocrystal structure of NKG2D and its class I MHC-like ligand ULBP3., Radaev S, Rostro B, Brooks AG, Colonna M, Sun PD, Immunity. 2001 Dec;15(6):1039-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11754823 11754823]
+
</div>
-
[[Category: Homo sapiens]]
+
<div class="pdbe-citations 1kcg" style="background-color:#fffaf0;"></div>
-
[[Category: Protein complex]]
+
-
[[Category: Radaev, S.]]
+
-
[[Category: Sun, P.]]
+
-
[[Category: GSH]]
+
-
[[Category: c-type lectin-like receptor]]
+
-
[[Category: mhc class i-like molecule]]
+
-
[[Category: protein-protein complex]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:49:52 2007''
+
==See Also==
 +
*[[NK cell receptor|NK cell receptor]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
 +
[[Category: Radaev S]]
 +
[[Category: Sun P]]

Current revision

NKG2D in complex with ULBP3

PDB ID 1kcg

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kcg"
Personal tools