5avo

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the reduced form of homoserine dehydrogenase from Sulfolobus tokodaii.

Structural highlights

5avo is a 2 chain structure with sequence from Sulfurisphaera tokodaii str. 7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DHOM_SULTO Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Homoserine dehydrogenase (HSD; 305 amino acid residues) catalyzes an NAD(P)-dependent reversible reaction between l-homoserine and aspartate 4-semialdehyde and is involved in the aspartate pathway. HSD from the hyperthermophilic archaeon Sulfolobus tokodaii was markedly activated (2.5-fold) by the addition of 0.8 mM dithiothreitol. The crystal structure of the homodimer indicated that the activation was caused by cleavage of the disulfide bond formed between two cysteine residues (C303) in the C-terminal regions of the two subunits.

Structural insight into activation of homoserine dehydrogenase from the archaeon Sulfolobus tokodaii via reduction.,Tomonaga Y, Kaneko R, Goto M, Ohshima T, Yoshimune K Biochem Biophys Rep. 2015 Jul 15;3:14-17. doi: 10.1016/j.bbrep.2015.07.006., eCollection 2015 Sep. PMID:29124164^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tomonaga Y, Kaneko R, Goto M, Ohshima T, Yoshimune K. Structural insight into activation of homoserine dehydrogenase from the archaeon Sulfolobus tokodaii via reduction. Biochem Biophys Rep. 2015 Jul 15;3:14-17. doi: 10.1016/j.bbrep.2015.07.006., eCollection 2015 Sep. PMID:29124164 doi:http://dx.doi.org/10.1016/j.bbrep.2015.07.006
↑ Ogata K, Yajima Y, Nakamura S, Kaneko R, Goto M, Ohshima T, Yoshimune K. Inhibition of homoserine dehydrogenase by formation of a cysteine-NAD covalent complex. Sci Rep. 2018 Apr 10;8(1):5749. doi: 10.1038/s41598-018-24063-1. PMID:29636528 doi:http://dx.doi.org/10.1038/s41598-018-24063-1
↑ Kubota T, Kurihara E, Watanabe K, Ogata K, Kaneko R, Goto M, Ohshima T, Yoshimune K. Conformational changes in the catalytic region are responsible for heat-induced activation of hyperthermophilic homoserine dehydrogenase. Commun Biol. 2022 Jul 14;5(1):704. doi: 10.1038/s42003-022-03656-7. PMID:35835834 doi:http://dx.doi.org/10.1038/s42003-022-03656-7
↑ Tomonaga Y, Kaneko R, Goto M, Ohshima T, Yoshimune K. Structural insight into activation of homoserine dehydrogenase from the archaeon Sulfolobus tokodaii via reduction. Biochem Biophys Rep. 2015 Jul 15;3:14-17. doi: 10.1016/j.bbrep.2015.07.006., eCollection 2015 Sep. PMID:29124164 doi:http://dx.doi.org/10.1016/j.bbrep.2015.07.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5avo"

Categories: Large Structures | Sulfurisphaera tokodaii str. 7 | Goto M | Kaneko R | Yoshimune K

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5avo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AVO FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5avo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5avo OCA], [https://pdbe.org/5avo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5avo RCSB], [https://www.ebi.ac.uk/pdbsum/5avo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5avo ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5avo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5avo OCA], [https://pdbe.org/5avo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5avo RCSB], [https://www.ebi.ac.uk/pdbsum/5avo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5avo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[https://www.uniprot.org/uniprot/F9VNG5_SULTO F9VNG5_SULTO]
+[https://www.uniprot.org/uniprot/DHOM_SULTO DHOM_SULTO] Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate.<ref>PMID:29124164</ref> <ref>PMID:29636528</ref> <ref>PMID:35835834</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

5avo

From Proteopedia

Current revision

Crystal structure of the reduced form of homoserine dehydrogenase from Sulfolobus tokodaii.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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