8p1y
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Arabidopsis thaliana mutated variant C244S of seryl-tRNA synthetase== | |
| + | <StructureSection load='8p1y' size='340' side='right'caption='[[8p1y]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8p1y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8P1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8P1Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8p1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8p1y OCA], [https://pdbe.org/8p1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8p1y RCSB], [https://www.ebi.ac.uk/pdbsum/8p1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8p1y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SYSC_ARATH SYSC_ARATH] Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).[UniProtKB:P0A8L1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have previously identified a unique disulfide bond in the crystal structure of Arabidopsis cytosolic seryl-tRNA synthetase involving cysteines evolutionarily conserved in all green plants. Here, we discovered that both cysteines are important for protein stability, but with opposite effects, and that their microenvironment may promote disulfide bond formation in oxidizing conditions. The crystal structure of the C244S mutant exhibited higher rigidity and an extensive network of noncovalent interactions correlating with its higher thermal stability. The activity of the wild-type showed resistance to oxidation with H(2) O(2) , while the activities of cysteine-to-serine mutants were impaired, indicating that the disulfide link may enable the protein to function under oxidative stress conditions which can be beneficial for an efficient plant stress response. | ||
| - | + | Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation.,Evic V, Soic R, Mocibob M, Kekez M, Houser J, Wimmerova M, Matkovic-Calogovic D, Gruic-Sovulj I, Kekez I, Rokov-Plavec J FEBS Lett. 2023 Dec;597(23):2975-2992. doi: 10.1002/1873-3468.14748. Epub 2023 , Oct 10. PMID:37804069<ref>PMID:37804069</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Kekez | + | <div class="pdbe-citations 8p1y" style="background-color:#fffaf0;"></div> |
| - | [[Category: Matkovic-Calogovic | + | == References == |
| - | [[Category: Rokov-Plavec | + | <references/> |
| - | [[Category: Soic | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kekez I]] | ||
| + | [[Category: Matkovic-Calogovic D]] | ||
| + | [[Category: Rokov-Plavec J]] | ||
| + | [[Category: Soic R]] | ||
Current revision
Arabidopsis thaliana mutated variant C244S of seryl-tRNA synthetase
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