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1l1c

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[[Image:1l1c.gif|left|200px]]
 
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==Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target==
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The line below this paragraph, containing "STRUCTURE_1l1c", creates the "Structure Box" on the page.
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<StructureSection load='1l1c' size='340' side='right'caption='[[1l1c]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l1c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L1C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l1c OCA], [https://pdbe.org/1l1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l1c RCSB], [https://www.ebi.ac.uk/pdbsum/1l1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l1c ProSAT]</span></td></tr>
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{{STRUCTURE_1l1c| PDB=1l1c | SCENE= }}
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</table>
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== Function ==
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'''Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target'''
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[https://www.uniprot.org/uniprot/LICT_BACSU LICT_BACSU] Mediates positive regulation of the glucanase operon (licST) by functioning as an antiterminator factor of transcription. Prevents termination at terminator lic-t.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/1l1c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l1c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
LicT is a bacterial regulatory protein able to prevent the premature arrest of transcription. When activated, LicT binds to a 29 base RNA hairpin overlapping a terminator located in the 5' mRNA leader region of the target genes. We have determined the solution structure of the LicT RNA-binding domain (CAT) in complex with its ribonucleic antiterminator (RAT) target by NMR spectroscopy (PDB 1L1C). CAT is a beta-stranded homodimer that undergoes no important conformational changes upon complex formation. It interacts, through mostly hydrophobic and stacking interactions, with the distorted minor groove of the hairpin stem that is interrupted by two asymmetric internal loops. Although different in sequence, these loops share sufficient structural analogy to be recognized similarly by symmetry-related elements of the protein dimer, leading to a quasi- symmetric structure reminiscent of that observed with dimeric transcription regulators bound to palindromic DNA. Sequence analysis suggests that this RNA- binding mode, where the RAT strands are clamped by the CAT dimer, is conserved in homologous systems.
LicT is a bacterial regulatory protein able to prevent the premature arrest of transcription. When activated, LicT binds to a 29 base RNA hairpin overlapping a terminator located in the 5' mRNA leader region of the target genes. We have determined the solution structure of the LicT RNA-binding domain (CAT) in complex with its ribonucleic antiterminator (RAT) target by NMR spectroscopy (PDB 1L1C). CAT is a beta-stranded homodimer that undergoes no important conformational changes upon complex formation. It interacts, through mostly hydrophobic and stacking interactions, with the distorted minor groove of the hairpin stem that is interrupted by two asymmetric internal loops. Although different in sequence, these loops share sufficient structural analogy to be recognized similarly by symmetry-related elements of the protein dimer, leading to a quasi- symmetric structure reminiscent of that observed with dimeric transcription regulators bound to palindromic DNA. Sequence analysis suggests that this RNA- binding mode, where the RAT strands are clamped by the CAT dimer, is conserved in homologous systems.
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==About this Structure==
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Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT.,Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M EMBO J. 2002 Apr 15;21(8):1987-97. PMID:11953318<ref>PMID:11953318</ref>
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1L1C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1C OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT., Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, EMBO J. 2002 Apr 15;21(8):1987-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11953318 11953318]
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</div>
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<div class="pdbe-citations 1l1c" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aymerich, S.]]
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[[Category: Aymerich S]]
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[[Category: Declerck, N.]]
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[[Category: Declerck N]]
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[[Category: Kochoyan, M.]]
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[[Category: Kochoyan M]]
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[[Category: Manival, X.]]
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[[Category: Manival X]]
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[[Category: Yang, Y.]]
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[[Category: Yang Y]]
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[[Category: Antiterminator complex]]
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[[Category: Protein rna complex]]
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[[Category: Rna hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:25:43 2008''
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Current revision

Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target

PDB ID 1l1c

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