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1l4a

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[[Image:1l4a.gif|left|200px]]
 
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==X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI==
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The line below this paragraph, containing "STRUCTURE_1l4a", creates the "Structure Box" on the page.
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<StructureSection load='1l4a' size='340' side='right'caption='[[1l4a]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l4a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L4A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l4a OCA], [https://pdbe.org/1l4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l4a RCSB], [https://www.ebi.ac.uk/pdbsum/1l4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l4a ProSAT]</span></td></tr>
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{{STRUCTURE_1l4a| PDB=1l4a | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYB_DORPE SYB_DORPE] Intrinsic membrane protein of small synaptic vesicles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l4/1l4a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l4a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nerve terminals release neurotransmitters from vesicles into the synaptic cleft upon transient increases in intracellular Ca(2+). This exocytotic process requires the formation of trans SNARE complexes and is regulated by accessory proteins including the complexins. Here we report the crystal structure of a squid core complexin-SNARE complex at 2.95-A resolution. A helical segment of complexin binds in anti-parallel fashion to the four-helix bundle of the core SNARE complex and interacts at its C terminus with syntaxin and synaptobrevin around the ionic zero layer of the SNARE complex. We propose that this structure is part of a multiprotein fusion machinery that regulates vesicle fusion at a late pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion.
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'''X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI'''
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X-ray structure of a neuronal complexin-SNARE complex from squid.,Bracher A, Kadlec J, Betz H, Weissenhorn W J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:12004067<ref>PMID:12004067</ref>
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==Overview==
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Nerve terminals release neurotransmitters from vesicles into the synaptic cleft upon transient increases in intracellular Ca(2+). This exocytotic process requires the formation of trans SNARE complexes and is regulated by accessory proteins including the complexins. Here we report the crystal structure of a squid core complexin-SNARE complex at 2.95-A resolution. A helical segment of complexin binds in anti-parallel fashion to the four-helix bundle of the core SNARE complex and interacts at its C terminus with syntaxin and synaptobrevin around the ionic zero layer of the SNARE complex. We propose that this structure is part of a multiprotein fusion machinery that regulates vesicle fusion at a late pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1L4A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Loligo_pealei Loligo pealei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L4A OCA].
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</div>
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<div class="pdbe-citations 1l4a" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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X-ray structure of a neuronal complexin-SNARE complex from squid., Bracher A, Kadlec J, Betz H, Weissenhorn W, J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12004067 12004067]
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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[[Category: Loligo pealei]]
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== References ==
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[[Category: Protein complex]]
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<references/>
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[[Category: Betz, H.]]
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__TOC__
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[[Category: Bracher, A.]]
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</StructureSection>
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[[Category: Kadlec, J.]]
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[[Category: Doryteuthis pealeii]]
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[[Category: Weissenhorn, W.]]
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[[Category: Large Structures]]
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[[Category: Membrane fusion]]
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[[Category: Betz H]]
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[[Category: Neurotransmission]]
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[[Category: Bracher A]]
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[[Category: Snare]]
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[[Category: Kadlec J]]
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[[Category: Snare complex]]
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[[Category: Weissenhorn W]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:31:38 2008''
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Current revision

X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI

PDB ID 1l4a

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