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2m5k

From Proteopedia

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Current revision

Atomic-resolution structure of a doublet cross-beta amyloid fibril

2m5k, resolution 12.70Å

Structural highlights

2m5k is a 8 chain structure with sequence from Rattus norvegicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy , Hybrid , Solid-state NMR, Resolution 12.7Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TTHY_RAT Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.^[1]

Publication Abstract from PubMed

The cross-beta amyloid form of peptides and proteins represents an archetypal and widely accessible structure consisting of ordered arrays of beta-sheet filaments. These complex aggregates have remarkable chemical and physical properties, and the conversion of normally soluble functional forms of proteins into amyloid structures is linked to many debilitating human diseases, including several common forms of age-related dementia. Despite their importance, however, cross-beta amyloid fibrils have proved to be recalcitrant to detailed structural analysis. By combining structural constraints from a series of experimental techniques spanning five orders of magnitude in length scale--including magic angle spinning nuclear magnetic resonance spectroscopy, X-ray fiber diffraction, cryoelectron microscopy, scanning transmission electron microscopy, and atomic force microscopy--we report the atomic-resolution (0.5 A) structures of three amyloid polymorphs formed by an 11-residue peptide. These structures reveal the details of the packing interactions by which the constituent beta-strands are assembled hierarchically into protofilaments, filaments, and mature fibrils.

Atomic structure and hierarchical assembly of a cross-beta amyloid fibril.,Fitzpatrick AW, Debelouchina GT, Bayro MJ, Clare DK, Caporini MA, Bajaj VS, Jaroniec CP, Wang L, Ladizhansky V, Muller SA, MacPhee CE, Waudby CA, Mott HR, De Simone A, Knowles TP, Saibil HR, Vendruscolo M, Orlova EV, Griffin RG, Dobson CM Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5468-73. doi:, 10.1073/pnas.1219476110. Epub 2013 Mar 19. PMID:23513222^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schreiber G, Aldred AR, Jaworowski A, Nilsson C, Achen MG, Segal MB. Thyroxine transport from blood to brain via transthyretin synthesis in choroid plexus. Am J Physiol. 1990 Feb;258(2 Pt 2):R338-45. PMID:2309926
↑ Fitzpatrick AW, Debelouchina GT, Bayro MJ, Clare DK, Caporini MA, Bajaj VS, Jaroniec CP, Wang L, Ladizhansky V, Muller SA, MacPhee CE, Waudby CA, Mott HR, De Simone A, Knowles TP, Saibil HR, Vendruscolo M, Orlova EV, Griffin RG, Dobson CM. Atomic structure and hierarchical assembly of a cross-beta amyloid fibril. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5468-73. doi:, 10.1073/pnas.1219476110. Epub 2013 Mar 19. PMID:23513222 doi:10.1073/pnas.1219476110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2m5k"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2m5k]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5K FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5k OCA], [https://pdbe.org/2m5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5k RCSB], [https://www.ebi.ac.uk/pdbsum/2m5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5k ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , Solid-state NMR, [[Resolution|Resolution]] 12.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5k OCA], [https://pdbe.org/2m5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5k RCSB], [https://www.ebi.ac.uk/pdbsum/2m5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5k ProSAT]</span></td></tr>
 </table>
 == Function ==

2m5k

From Proteopedia

Current revision

Atomic-resolution structure of a doublet cross-beta amyloid fibril

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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