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1kps

From Proteopedia

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(New page: 200px<br /> <applet load="1kps" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kps, resolution 2.50&Aring;" /> '''Structural Basis fo...)
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[[Image:1kps.gif|left|200px]]<br />
 
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<applet load="1kps" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kps, resolution 2.50&Aring;" />
 
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'''Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1'''<br />
 
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==Overview==
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==Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1==
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E2 enzymes catalyze attachment of ubiquitin and ubiquitin-like proteins to, lysine residues directly or through E3-mediated reactions. The small, ubiquitin-like modifier SUMO regulates nuclear transport, stress response, and signal transduction in eukaryotes and is essential for cell-cycle, progression in yeast. In contrast to most ubiquitin conjugation, the SUMO, E2 enzyme Ubc9 is sufficient for substrate recognition and lysine, modification of known SUMO targets. Crystallographic analysis of a complex, between mammalian Ubc9 and a C-terminal domain of RanGAP1 at 2.5 A reveals, structural determinants for recognition of consensus SUMO modification, sequences found within SUMO-conjugated proteins. Structure-based, mutagenesis and biochemical analysis of Ubc9 and RanGAP1 reveal distinct, motifs required for substrate binding and SUMO modification of p53, IkappaBalpha, and RanGAP1.
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<StructureSection load='1kps' size='340' side='right'caption='[[1kps]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kps]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KPS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kps OCA], [https://pdbe.org/1kps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kps RCSB], [https://www.ebi.ac.uk/pdbsum/1kps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kps ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kp/1kps_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kps ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KPS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KPS OCA].
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*[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]]
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== References ==
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==Reference==
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<references/>
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Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1., Bernier-Villamor V, Sampson DA, Matunis MJ, Lima CD, Cell. 2002 Feb 8;108(3):345-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11853669 11853669]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Bernier-Villamor V]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Lima CD]]
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[[Category: Bernier-Villamor, V.]]
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[[Category: Matunis MJ]]
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[[Category: Lima, C.D.]]
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[[Category: Sampson DA]]
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[[Category: Matunis, M.J.]]
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[[Category: Sampson, D.A.]]
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[[Category: SO4]]
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[[Category: conjugating enzyme]]
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[[Category: e2]]
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[[Category: ligase]]
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[[Category: small ubiquitin-like modifier]]
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[[Category: sumo]]
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[[Category: thioester]]
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[[Category: ubiquitin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:53:31 2007''
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Current revision

Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1

PDB ID 1kps

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