1l7d

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[[Image:1l7d.gif|left|200px]]
 
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==Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)==
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The line below this paragraph, containing "STRUCTURE_1l7d", creates the "Structure Box" on the page.
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<StructureSection load='1l7d' size='340' side='right'caption='[[1l7d]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l7d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L7D FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l7d OCA], [https://pdbe.org/1l7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l7d RCSB], [https://www.ebi.ac.uk/pdbsum/1l7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l7d ProSAT]</span></td></tr>
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{{STRUCTURE_1l7d| PDB=1l7d | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PNTAA_RHORT PNTAA_RHORT] The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.[UniProtKB:P07001]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l7/1l7d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l7d ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)'''
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==See Also==
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*[[NAD(P) transhydrogenase 3D structures|NAD(P) transhydrogenase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Transhydrogenase (TH) is a dimeric integral membrane enzyme in mitochondria and prokaryotes that couples proton translocation across a membrane with hydride transfer between NAD(H) and NADP(H) in soluble domains. Crystal structures of the NAD(H) binding alpha1 subunit (domain I) of Rhodospirillum rubrum TH have been determined at 1.8 A resolution in the absence of dinucleotide and at 1.9 A resolution with NADH bound. Each structure contains two domain I dimers in the asymmetric unit (AB and CD); the dimers are intimately associated and related by noncrystallographic 2-fold axes. NADH binds to subunits A and D, consistent with the half-of-the-sites reactivity of the enzyme. The conformation of NADH in subunits A and D is very similar; the nicotinamide is in the anti conformation, the A-face is exposed to solvent, and both N7 and O7 participate in hydrogen bonds. Comparison of subunits A and D to six independent copies of the subunit without bound NADH reveals multiple conformations for residues and loops surrounding the NADH site, indicating flexibility for binding and release of the substrate (product). The NADH-bound structure is also compared to the structures of R. rubrum domain I with NAD bound (PDB code 1F8G) and with NAD bound in complex with domain III of TH (PDB code 1HZZ). The NADH- vs NAD-bound domain I structures reveal conformational differences in conserved residues in the NAD(H) binding site and in dinucleotide conformation that are correlated with the net charge, i.e., oxidation state, of the nicotinamides. The comparisons illustrate how nicotinamide oxidation state can affect the domain I conformation, which is relevant to the hydride transfer step of the overall reaction.
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[[Category: Large Structures]]
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==About this Structure==
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1L7D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7D OCA].
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==Reference==
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Crystal structures of transhydrogenase domain I with and without bound NADH., Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD, Biochemistry. 2002 Oct 22;41(42):12745-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379117 12379117]
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[[Category: Rhodospirillum rubrum]]
[[Category: Rhodospirillum rubrum]]
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[[Category: Single protein]]
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[[Category: Hatefi Y]]
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[[Category: Hatefi, Y.]]
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[[Category: Prasad GS]]
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[[Category: Prasad, G S.]]
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[[Category: Sridhar V]]
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[[Category: Sridhar, V.]]
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[[Category: Stout CD]]
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[[Category: Stout, C D.]]
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[[Category: Wahlberg M]]
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[[Category: Wahlberg, M.]]
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[[Category: Yamaguchi M]]
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[[Category: Yamaguchi, M.]]
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[[Category: Transhydrogenase domain i]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:37:42 2008''
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Current revision

Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)

PDB ID 1l7d

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