6yy4

<table><tr><td colspan='2'>[[6yy4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YY4 FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yy4 OCA], [https://pdbe.org/6yy4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yy4 RCSB], [https://www.ebi.ac.uk/pdbsum/6yy4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yy4 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

G-quadruplexes are noncanonical nucleic acid structures formed by stacked guanine tetrads. They are capable of a range of functions and thought to play widespread biological roles. This diversity raises an important question: what determines the biochemical specificity of G-quadruplex structures? The answer is particularly important from the perspective of biological regulation because genomes can contain hundreds of thousands of G-quadruplexes with a range of functions. Here we analyze the specificity of each sequence in a 496-member library of variants of a reference G-quadruplex with respect to five functions. Our analysis shows that the sequence requirements of G-quadruplexes with these functions are different from one another, with some mutations altering biochemical specificity by orders of magnitude. Mutations in tetrads have larger effects than mutations in loops, and changes in specificity are correlated with changes in multimeric state. To complement our biochemical data we determined the solution structure of a monomeric G-quadruplex from the library. The stacked and accessible tetrads rationalize why monomers tend to promote a model peroxidase reaction and generate fluorescence. Our experiments support a model in which the sequence requirements of G-quadruplexes with different functions are overlapping but distinct. This has implications for biological regulation, bioinformatics, and drug design.

Overlapping but distinct: a new model for G-quadruplex biochemical specificity.,Volek M, Kolesnikova S, Svehlova K, Srb P, Sgallova R, Streckerova T, Redondo JA, Veverka V, Curtis EA Nucleic Acids Res. 2021 Feb 26;49(4):1816-1827. doi: 10.1093/nar/gkab037. PMID:33544841<ref>PMID:33544841</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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[[Category: Synthetic construct]]