1l7j

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[[Image:1l7j.gif|left|200px]]
 
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==X-ray structure of galactose mutarotase from Lactococcus lactis (apo)==
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The line below this paragraph, containing "STRUCTURE_1l7j", creates the "Structure Box" on the page.
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<StructureSection load='1l7j' size='340' side='right'caption='[[1l7j]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L7J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l7j OCA], [https://pdbe.org/1l7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l7j RCSB], [https://www.ebi.ac.uk/pdbsum/1l7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l7j ProSAT]</span></td></tr>
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{{STRUCTURE_1l7j| PDB=1l7j | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9ZB17_9LACT Q9ZB17_9LACT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l7/1l7j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l7j ConSurf].
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<div style="clear:both"></div>
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'''X-ray structure of galactose mutarotase from Lactococcus lactis (apo)'''
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==See Also==
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*[[Galactose mutarotase|Galactose mutarotase]]
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__TOC__
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==Overview==
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</StructureSection>
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Galactose mutarotase plays a key role in normal galactose metabolism by catalyzing the interconversion of beta-D-galactose and alpha-D-galactose. Here we describe the three-dimensional architecture of galactose mutarotase from Lactococcus lactis determined to 1.9-A resolution. Each subunit of the dimeric enzyme displays a distinctive beta-sandwich motif. This tertiary structural element was first identified in beta-galactosidase and subsequently observed in copper amine oxidase, hyaluronate lyase, chondroitinase, and maltose phosphorylase. Two cis-peptides are found in each subunit, namely Pro(67) and Lys(136). The active site is positioned in a rather open cleft, and the electron density corresponding to the bound galactose unequivocally demonstrates that both anomers of the substrate are present in the crystalline enzyme. Those residues responsible for anchoring the sugar to the protein include Arg(71), His(96), His(170), Asp(243), and Glu(304). Both His(96) and His(170) are strictly conserved among mutarotase amino acid sequences determined thus far. The imidazole nitrogens of these residues are located within hydrogen bonding distance to the C-5 oxygen of galactose. Strikingly, the carboxylate group of Glu(304) is situated at approximately 2.7 A from the 1'-hydroxyl group of galactose, thereby suggesting its possible role as a general acid/base group.
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==About this Structure==
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1L7J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L7J OCA].
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==Reference==
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High resolution X-ray structure of galactose mutarotase from Lactococcus lactis., Thoden JB, Holden HM, J Biol Chem. 2002 Jun 7;277(23):20854-61. Epub 2002 Mar 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11907040 11907040]
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[[Category: Aldose 1-epimerase]]
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[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Holden, H M.]]
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[[Category: Holden HM]]
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[[Category: Thoden, J B.]]
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[[Category: Thoden JB]]
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[[Category: Epimerase]]
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[[Category: Galactose metabolism]]
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[[Category: Mutarotase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:37:57 2008''
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Current revision

X-ray structure of galactose mutarotase from Lactococcus lactis (apo)

PDB ID 1l7j

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