8jq9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Gabija GajA== | |
| + | <StructureSection load='8jq9' size='340' side='right'caption='[[8jq9]], [[Resolution|resolution]] 2.66Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8jq9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_VD045 Bacillus cereus VD045]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JQ9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.66Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jq9 OCA], [https://pdbe.org/8jq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jq9 RCSB], [https://www.ebi.ac.uk/pdbsum/8jq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jq9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Prokaryotes have evolved intricate innate immune systems against phage infection(1-7). Gabija is a highly widespread prokaryotic defence system that consists of two components, GajA and GajB(8). GajA functions as a DNA endonuclease that is inactive in the presence of ATP(9). Here, to explore how the Gabija system is activated for anti-phage defence, we report its cryo-electron microscopy structures in five states, including apo GajA, GajA in complex with DNA, GajA bound by ATP, apo GajA-GajB, and GajA-GajB in complex with ATP and Mg(2+). GajA is a rhombus-shaped tetramer with its ATPase domain clustered at the centre and the topoisomerase-primase (Toprim) domain located peripherally. ATP binding at the ATPase domain stabilizes the insertion region within the ATPase domain, keeping the Toprim domain in a closed state. Upon ATP depletion by phages, the Toprim domain opens to bind and cleave the DNA substrate. GajB, which docks on GajA, is activated by the cleaved DNA, ultimately leading to prokaryotic cell death. Our study presents a mechanistic landscape of Gabija activation. | ||
| - | + | Structures and activation mechanism of the Gabija anti-phage system.,Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L Nature. 2024 May;629(8011):467-473. doi: 10.1038/s41586-024-07270-x. Epub 2024 , Mar 12. PMID:38471529<ref>PMID:38471529</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8jq9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus cereus VD045]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Li J]] | ||
| + | [[Category: Wang L]] | ||
| + | [[Category: Wang Z]] | ||
Current revision
Structure of Gabija GajA
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