8p1g

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'''Unreleased structure'''
 
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The entry 8p1g is ON HOLD until Paper Publication
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==Crystal structure of inactive TtCE16 in complex with acetate==
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<StructureSection load='8p1g' size='340' side='right'caption='[[8p1g]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8p1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus Thermothelomyces thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8P1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8P1G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8p1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8p1g OCA], [https://pdbe.org/8p1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8p1g RCSB], [https://www.ebi.ac.uk/pdbsum/8p1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8p1g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/G2QL32_THET4 G2QL32_THET4]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acetyl esterases belonging to the carbohydrate esterase family 16 (CE16) is a growing group of enzymes, with exceptional diversity regarding substrate specificity and regioselectivity. However, further insight into the CE16 specificity is required for their efficient biotechnological exploitation. In this work, exo-deacetylase TtCE16B from Thermothelomyces thermophila was heterologously expressed and biochemically characterized. The esterase targets positions O-3 and O-4 of singly and doubly acetylated non-reducing-end xylopyranosyl residues, provided the presence of a free vicinal hydroxyl group at position O-4 and O-3, respectively. Crystal structure of TtCE16B, the first representative among the CE16 enzymes, in apo- and product-bound form, allowed the identification of residues forming the catalytic triad and oxyanion hole, as well as the structural elements related to the enzyme preference for oligomers. The role of TtCE16B in hemicellulose degradation was investigated on acetylated xylan from birchwood and pre-treated beechwood biomass. TtCE16B exhibited complementary activity to commercially available OCE6 acetylxylan esterase. Moreover, it showed synergistic effects with SrXyl43 beta-xylosidase. Overall, supplementation of xylan-targeting enzymatic mixtures with both TtCE16B and OCE6 esterases led to a 3-fold or 4-fold increase in xylose release, when using TmXyn10 and TtXyn30A xylanases respectively.
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Authors: Dimarogona, M., Kosinas, C., Pentari, C., Zerva, A., Topakas, E., Karampa, P.
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The role of CE16 exo-deacetylases in hemicellulolytic enzyme mixtures revealed by the biochemical and structural study of the novel TtCE16B esterase.,Pentari C, Zerva A, Kosinas C, Karampa P, Puchart V, Dimarogona M, Topakas E Carbohydr Polym. 2024 Mar 1;327:121667. doi: 10.1016/j.carbpol.2023.121667. Epub , 2023 Dec 7. PMID:38171682<ref>PMID:38171682</ref>
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Description: Crystal structure of inactive TtCE16 in complex with acetate
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Topakas, E]]
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<div class="pdbe-citations 8p1g" style="background-color:#fffaf0;"></div>
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[[Category: Pentari, C]]
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== References ==
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[[Category: Kosinas, C]]
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<references/>
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[[Category: Zerva, A]]
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__TOC__
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[[Category: Dimarogona, M]]
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</StructureSection>
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[[Category: Karampa, P]]
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[[Category: Large Structures]]
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[[Category: Thermothelomyces thermophilus]]
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[[Category: Dimarogona M]]
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[[Category: Karampa P]]
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[[Category: Kosinas C]]
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[[Category: Pentari C]]
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[[Category: Topakas E]]
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[[Category: Zerva A]]

Current revision

Crystal structure of inactive TtCE16 in complex with acetate

PDB ID 8p1g

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