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1lah

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[[Image:1lah.gif|left|200px]]
 
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==STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN==
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The line below this paragraph, containing "STRUCTURE_1lah", creates the "Structure Box" on the page.
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<StructureSection load='1lah' size='340' side='right'caption='[[1lah]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lah]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LAH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>
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{{STRUCTURE_1lah| PDB=1lah | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lah FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lah OCA], [https://pdbe.org/1lah PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lah RCSB], [https://www.ebi.ac.uk/pdbsum/1lah PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lah ProSAT]</span></td></tr>
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</table>
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'''STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN'''
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== Function ==
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[https://www.uniprot.org/uniprot/ARGT_SALTY ARGT_SALTY] This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (HisJ) interact with a common membrane-bound receptor, HisP.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The substrate-binding site of a protein with multiple specificity should satisfy geometric and energetic complementarity for several different substrates. The structural basis of the multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (LAO) was investigated by determining and analyzing the structures of the protein unliganded and liganded with each of the three high-affinity ligands (L-lysine, L-arginine, and L-ornithine) and with one low-affinity ligand (L-histidine). The geometric complementarity is achieved primarily by virtue of the large size of the ligand-binding site which can accommodate the maximum common volume of the four ligands plus three water molecules. The optimization of energetic complementarity is achieved by the relocation of protein-bound water molecules and by the movement of the Asp-11 side chain. The structure of the LAO-histidine complex indicates that the 30-fold reduced affinity of the protein for histidine is primarily due to unavailability of one ionic interaction of the histidine side chain with the protein which is present in the other three complexes.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/1lah_consurf.spt"</scriptWhenChecked>
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1LAH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAH OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural basis for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein., Oh BH, Ames GF, Kim SH, J Biol Chem. 1994 Oct 21;269(42):26323-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7929349 7929349]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lah ConSurf].
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[[Category: Salmonella typhimurium]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Kim, S H.]]
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</StructureSection>
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[[Category: Oh, B H.]]
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[[Category: Large Structures]]
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[[Category: Amino acid transport]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:43:35 2008''
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[[Category: Kim S-H]]
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[[Category: Oh B-H]]

Current revision

STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN

PDB ID 1lah

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