1lay

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[[Image:1lay.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF CYTOMEGALOVIRUS PROTEASE==
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The line below this paragraph, containing "STRUCTURE_1lay", creates the "Structure Box" on the page.
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<StructureSection load='1lay' size='340' side='right'caption='[[1lay]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lay]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LAY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lay OCA], [https://pdbe.org/1lay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lay RCSB], [https://www.ebi.ac.uk/pdbsum/1lay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lay ProSAT]</span></td></tr>
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{{STRUCTURE_1lay| PDB=1lay | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SCAF_HCMVA SCAF_HCMVA] Capsid scaffolding protein acts as a scaffold protein by binding major capsid protein UL86 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as protein UL86 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein UL86. Cleavages products are evicted from the capsid before or during DNA packaging (By similarity). Assemblin is a protease essential for virion assembly in the nucleus. Catalyzes the cleavage of the assembly protein after complete capsid formation. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging (By similarity). Assembly protein plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein UL86. Multimerizes in the nucleus such as protein UL86 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/1lay_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lay ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF CYTOMEGALOVIRUS PROTEASE'''
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==See Also==
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Human herpesviruses are responsible for a variety of diseases. They are divided into three subfamilies: alpha includes herpes simplex viruses (HSV-1 and HSV-2) and varicella-zoster virus (VZV); beta includes cytomegalovirus (CMV) and human herpesvirus-6 (HHV-6); and gamma includes Epstein-Barr virus (EBV). Each virus encodes a serine protease that is essential for its replication and is a potential target for therapeutic intervention. Human CMV is a ubiquitous opportunistic pathogen that can result in life-threatening infections in congenitally infected infants, immunocompromised individuals and immunosuppressed cancer or transplant patients. Here we report the crystal structure of human CMV protease at 2.5 angstroms resolution. The structure reveals a fold that has not been reported for any other serine protease, and an active site consisting of a novel catalytic triad in which the third member is a histidine instead of an aspartic acid, or possibly a catalytic tetrad consisting of a serine, two histidines and an aspartic acid. An unusual dimer interface that is important to the protease activity has also been identified.
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==About this Structure==
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1LAY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAY OCA].
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==Reference==
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Unique fold and active site in cytomegalovirus protease., Qiu X, Culp JS, DiLella AG, Hellmig B, Hoog SS, Janson CA, Smith WW, Abdel-Meguid SS, Nature. 1996 Sep 19;383(6597):275-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8805707 8805707]
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[[Category: Cytomegalovirus]]
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[[Category: Single protein]]
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[[Category: Abdel-Meguid, S S.]]
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[[Category: Culp, J S.]]
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[[Category: Dilella, A G.]]
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[[Category: Hellmig, B.]]
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[[Category: Hoog, S S.]]
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[[Category: Jason, C A.]]
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[[Category: Qiu, X.]]
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[[Category: Smith, W W.]]
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[[Category: Cytomegalovirus]]
[[Category: Cytomegalovirus]]
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[[Category: Serine protease]]
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[[Category: Large Structures]]
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[[Category: Viral protease]]
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[[Category: Abdel-Meguid SS]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:44:15 2008''
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[[Category: Culp JS]]
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[[Category: Dilella AG]]
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[[Category: Hellmig B]]
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[[Category: Hoog SS]]
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[[Category: Jason CA]]
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[[Category: Qiu X]]
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[[Category: Smith WW]]

Current revision

CRYSTAL STRUCTURE OF CYTOMEGALOVIRUS PROTEASE

PDB ID 1lay

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