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| - | [[Image:1lcw.gif|left|200px]] | |
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| - | <!--
| + | ==streptavidin-homobiotin complex== |
| - | The line below this paragraph, containing "STRUCTURE_1lcw", creates the "Structure Box" on the page.
| + | <StructureSection load='1lcw' size='340' side='right'caption='[[1lcw]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1lcw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCW FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SHM:HOMOBIOTIN'>SHM</scene></td></tr> |
| - | {{STRUCTURE_1lcw| PDB=1lcw | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcw OCA], [https://pdbe.org/1lcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lcw RCSB], [https://www.ebi.ac.uk/pdbsum/1lcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcw ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lcw_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lcw ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''streptavidin-homobiotin complex'''
| + | ==See Also== |
| - | | + | *[[Avidin 3D structures|Avidin 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | We have studied the structural elements that affect ligand exchange between the two high affinity biotin-binding proteins, egg white avidin and its bacterial analogue, streptavidin. For this purpose, we have developed a simple assay based on the antipodal behavior of the two proteins toward hydrolysis of biotinyl p-nitrophenyl ester (BNP). The assay provided the experimental basis for these studies. It was found that biotin migrates unidirectionally from streptavidin to avidin. Conversely, the biotin derivative, BNP, is transferred in the opposite direction, from avidin to streptavidin. A previous crystallographic study (Huberman, T., Eisenberg-Domovich, Y., Gitlin, G., Kulik, T., Bayer, E. A., Wilchek, M., and Livnah, O. (2001) J. Biol. Chem. 276, 32031-32039) provided insight into a plausible explanation for these results. These data revealed that the non-hydrolyzable BNP analogue, biotinyl p-nitroanilide, was almost completely sheltered in streptavidin as opposed to avidin in which the disordered conformation of a critical loop resulted in the loss of several hydrogen bonds and concomitant exposure of the analogue to the solvent. In order to determine the minimal modification of the biotin molecule required to cause the disordered loop conformation, the structures of avidin and streptavidin were determined with norbiotin, homobiotin, and a common long-chain biotin derivative, biotinyl epsilon-aminocaproic acid. Six new crystal structures of the avidin and streptavidin complexes with the latter biotin analogues and derivatives were thus elucidated. It was found that extending the biotin side chain by a single CH(2) group (i.e. homobiotin) is sufficient to result in this remarkable conformational change in the loop of avidin. These results bear significant biotechnological importance, suggesting that complexes containing biotinylated probes with streptavidin would be more stable than those with avidin. These findings should be heeded when developing new drugs based on lead compounds because it is difficult to predict the structural and conformational consequences on the resultant protein-ligand interactions.
| + | [[Category: Large Structures]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1LCW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCW OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin., Pazy Y, Kulik T, Bayer EA, Wilchek M, Livnah O, J Biol Chem. 2002 Aug 23;277(34):30892-900. Epub 2002 Jun 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12055191 12055191]
| + | |
| - | [[Category: Single protein]] | + | |
| | [[Category: Streptomyces avidinii]] | | [[Category: Streptomyces avidinii]] |
| - | [[Category: Bayer, E A.]] | + | [[Category: Bayer EA]] |
| - | [[Category: Livnah, O.]] | + | [[Category: Livnah O]] |
| - | [[Category: Pazy, Y.]] | + | [[Category: Pazy Y]] |
| - | [[Category: Wilchek, M.]] | + | [[Category: Wilchek M]] |
| - | [[Category: Avidin]]
| + | |
| - | [[Category: Biotin]]
| + | |
| - | [[Category: High affinity system]]
| + | |
| - | [[Category: Ligand exchange]]
| + | |
| - | [[Category: Streptavidin]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:47:45 2008''
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