1lf1

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[[Image:1lf1.gif|left|200px]]
 
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==Crystal Structure of Cel5 from Alkalophilic Bacillus sp.==
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The line below this paragraph, containing "STRUCTURE_1lf1", creates the "Structure Box" on the page.
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<StructureSection load='1lf1' size='340' side='right'caption='[[1lf1]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lf1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LF1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lf1 OCA], [https://pdbe.org/1lf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lf1 RCSB], [https://www.ebi.ac.uk/pdbsum/1lf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lf1 ProSAT]</span></td></tr>
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{{STRUCTURE_1lf1| PDB=1lf1 | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of Cel5 from Alkalophilic Bacillus sp.'''
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[https://www.uniprot.org/uniprot/Q59232_BACSP Q59232_BACSP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The crystal structure of an alkaline Bacillus cellulase catalytic core, from glucoside hydrolase family 5, reveals a novel combination of the catalytic machinery of two classic textbook enzymes. The enzyme has the expected two glutamate residues in close proximity to one another in the active-site that are typical of retaining cellulases. However, the proton donor, glutamate 139 is also unexpectedly a member of a serine-histidine-glutamate catalytic triad, forming a novel combination of catalytic machineries. Structure and sequence analysis of glucoside hydrolase family 5 reveal that the triad is highly conserved, but with variations at the equivalent of the serine position. We speculate that the purpose of this novel catalytic triad is to control the protonation of the acid/base glutamate, facilitating the first step of the catalytic reaction, protonation of the substrate, by the proton donor glutamate. If correct, this will be a novel use for a catalytic triad.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lf/1lf1_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1LF1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF1 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lf1 ConSurf].
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A novel combination of two classic catalytic schemes., Shaw A, Bott R, Vonrhein C, Bricogne G, Power S, Day AG, J Mol Biol. 2002 Jul 5;320(2):303-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079387 12079387]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Cellulase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bott R]]
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[[Category: Bott, R.]]
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[[Category: Bricogne G]]
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[[Category: Bricogne, G.]]
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[[Category: Day AG]]
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[[Category: Day, A G.]]
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[[Category: Power S]]
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[[Category: Power, S.]]
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[[Category: Shaw A]]
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[[Category: Shaw, A.]]
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[[Category: Vonrhein C]]
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[[Category: Vonrhein, C.]]
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[[Category: Cellulose degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:51:34 2008''
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Current revision

Crystal Structure of Cel5 from Alkalophilic Bacillus sp.

PDB ID 1lf1

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