8clk
From Proteopedia
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== Function == | == Function == | ||
| - | [https://www.uniprot.org/uniprot/ | + | [https://www.uniprot.org/uniprot/TF3C6_HUMAN TF3C6_HUMAN] Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.<ref>PMID:17409385</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules tauA and tauB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The tauB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both tauA and tauB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation. | ||
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| + | Structural insights into human TFIIIC promoter recognition.,Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW Sci Adv. 2023 Jul 7;9(27):eadh2019. doi: 10.1126/sciadv.adh2019. Epub 2023 Jul 7. PMID:37418517<ref>PMID:37418517</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8clk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Eustermann S]] |
| - | [[Category: | + | [[Category: Girbig M]] |
| - | [[Category: | + | [[Category: Hauptmann L]] |
| - | [[Category: | + | [[Category: Hoffmann T]] |
| - | [[Category: | + | [[Category: Mueller CW]] |
| - | [[Category: | + | [[Category: Seifert-Davila W]] |
Current revision
TFIIIC TauA complex
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