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1oek

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(New page: 200px<br /> <applet load="1oek" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oek, resolution 2.4&Aring;" /> '''YODA FROM ESCHERICHI...)
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[[Image:1oek.gif|left|200px]]<br />
 
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<applet load="1oek" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oek, resolution 2.4&Aring;" />
 
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'''YODA FROM ESCHERICHIA COLI CRYSTALLISED WITH ZINC IONS'''<br />
 
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==Overview==
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==YodA from Escherichia coli crystallised with zinc ions==
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We have determined the crystal structure of YodA, an Escherichia coli, protein of unknown function. YodA had been identified under conditions of, cadmium stress, and we confirm that it binds metals such as cadmium and, zinc. We have also found nickel bound in one of the crystal forms. YodA is, composed of two domains: a main lipocalin/calycin-like domain and a, helical domain. The principal metal-binding site lies on one side of the, calycin domain, thus making YodA the first metal-binding lipocalin known., Our experiments suggest that YodA expression may be part of a more general, stress response. From sequence analogy with the C-terminal domain of a, metal-binding receptor of a member of bacterial ATP-binding cassette, transporters, we propose a three-dimensional model for this receptor ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12909634 (full description)]]
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<StructureSection load='1oek' size='340' side='right'caption='[[1oek]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oek]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OEK FirstGlance]. <br>
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1OEK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OEK OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oek OCA], [https://pdbe.org/1oek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oek RCSB], [https://www.ebi.ac.uk/pdbsum/1oek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oek ProSAT]</span></td></tr>
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YodA from Escherichia coli is a metal-binding, lipocalin-like protein., David G, Blondeau K, Schiltz M, Penel S, Lewit-Bentley A, J Biol Chem. 2003 Oct 31;278(44):43728-35. Epub 2003 Aug 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12909634 12909634]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ZINT_ECOLI ZINT_ECOLI] May function as a periplasmic zinc chaperone or mediate direct transport of zinc from the periplasm to the cytoplasm under zinc-limited conditions. Binds zinc with high affinity, and can also bind cadmium, mercury or nickel. Preferentially binds Zn(2+) over Cd(2+). Contains one high affinity metal binding site, and can bind additional metal ions at other sites.<ref>PMID:17931600</ref> <ref>PMID:19377097</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oe/1oek_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oek ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Blondeau, K.]]
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[[Category: Blondeau K]]
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[[Category: David, G.]]
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[[Category: David G]]
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[[Category: Lewit-Bentley, A.]]
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[[Category: Lewit-Bentley A]]
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[[Category: Penel, S.]]
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[[Category: Penel S]]
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[[Category: Renouard, M.]]
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[[Category: Renouard M]]
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[[Category: ZN]]
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[[Category: lipocalin]]
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[[Category: stress protein]]
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[[Category: yoda]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:17:54 2007''
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Current revision

YodA from Escherichia coli crystallised with zinc ions

PDB ID 1oek

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