1lar

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(New page: 200px<br /> <applet load="1lar" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lar, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1lar.gif|left|200px]]<br />
 
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<applet load="1lar" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lar, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE TANDEM PHOSPHATASE DOMAINS OF RPTP LAR'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE TANDEM PHOSPHATASE DOMAINS OF RPTP LAR==
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Most receptor-like protein tyrosine phosphatases (RPTPs) contain two, conserved phosphatase domains (D1 and D2) in their intracellular region., The carboxy-terminal D2 domain has little or no catalytic activity. The, crystal structure of the tandem D1 and D2 domains of the human RPTP LAR, revealed that the tertiary structures of the LAR D1 and D2 domains are, very similar to each other, with the exception of conformational, differences at two amino acid positions in the D2 domain. Site-directed, mutational changes at these positions (Leu-1644-to-Tyr and, Glu-1779-to-Asp) conferred a robust PTPase activity to the D2 domain. The, catalytic sites of both domains are accessible, in contrast to the dimeric, blocked orientation model previously suggested. The relative orientation, of the LAR D1 and D2 domains, constrained by a short linker, is stabilized, by extensive interdomain interactions, suggesting that this orientation, might be favored in solution.
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<StructureSection load='1lar' size='340' side='right'caption='[[1lar]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lar]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LAR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lar OCA], [https://pdbe.org/1lar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lar RCSB], [https://www.ebi.ac.uk/pdbsum/1lar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lar ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTPRF_HUMAN PTPRF_HUMAN] Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase).<ref>PMID:10338209</ref> The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.<ref>PMID:10338209</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/1lar_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lar ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Most receptor-like protein tyrosine phosphatases (RPTPs) contain two conserved phosphatase domains (D1 and D2) in their intracellular region. The carboxy-terminal D2 domain has little or no catalytic activity. The crystal structure of the tandem D1 and D2 domains of the human RPTP LAR revealed that the tertiary structures of the LAR D1 and D2 domains are very similar to each other, with the exception of conformational differences at two amino acid positions in the D2 domain. Site-directed mutational changes at these positions (Leu-1644-to-Tyr and Glu-1779-to-Asp) conferred a robust PTPase activity to the D2 domain. The catalytic sites of both domains are accessible, in contrast to the dimeric blocked orientation model previously suggested. The relative orientation of the LAR D1 and D2 domains, constrained by a short linker, is stabilized by extensive interdomain interactions, suggesting that this orientation might be favored in solution.
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==Disease==
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Crystal structure of the tandem phosphatase domains of RPTP LAR.,Nam HJ, Poy F, Krueger NX, Saito H, Frederick CA Cell. 1999 May 14;97(4):449-57. PMID:10338209<ref>PMID:10338209</ref>
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Known disease associated with this structure: Leukemia, acute myeloid OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604763 604763]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1LAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LAR OCA].
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</div>
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<div class="pdbe-citations 1lar" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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Crystal structure of the tandem phosphatase domains of RPTP LAR., Nam HJ, Poy F, Krueger NX, Saito H, Frederick CA, Cell. 1999 May 14;97(4):449-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10338209 10338209]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Frederick CA]]
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[[Category: Frederick, C.A.]]
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[[Category: Krueger N]]
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[[Category: Krueger, N.]]
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[[Category: Nam H-J]]
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[[Category: Nam, H.J.]]
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[[Category: Poy F]]
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[[Category: Poy, F.]]
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[[Category: Saito H]]
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[[Category: Saito, H.]]
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[[Category: lar protein]]
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[[Category: tyrosine phosphatease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:58:14 2007''
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Current revision

CRYSTAL STRUCTURE OF THE TANDEM PHOSPHATASE DOMAINS OF RPTP LAR

PDB ID 1lar

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