8ps4
From Proteopedia
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(New page: '''Unreleased structure''' The entry 8ps4 is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Escherichia coli SduA complex== | |
| + | <StructureSection load='8ps4' size='340' side='right'caption='[[8ps4]], [[Resolution|resolution]] 2.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ps4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_KTE10 Escherichia coli KTE10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PS4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.95Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ps4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ps4 OCA], [https://pdbe.org/8ps4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ps4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ps4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ps4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host defense mechanisms in innate anti-phage immunity. Shedu is a single-component defense system comprising a putative nuclease SduA. Here, we report cryoelectron microscopy (cryo-EM) structures of apo- and double-stranded DNA (dsDNA)-bound tetrameric SduA assemblies, revealing that the N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD-(D/E)XK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 5' end. The end-directed DNA nicking activity of Shedu prevents propagation of linear DNA in vivo. Finally, we show that phages escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway. Taken together, these results define the antiviral mechanism of Shedu systems, underlining the paradigm that recognition of pathogen-specific nucleic acid structures is a conserved feature of innate immunity across all domains of life. | ||
| - | + | DNA end sensing and cleavage by the Shedu anti-phage defense system.,Loeff L, Walter A, Rosalen GT, Jinek M Cell. 2024 Dec 30:S0092-8674(24)01346-1. doi: 10.1016/j.cell.2024.11.030. PMID:39742808<ref>PMID:39742808</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8ps4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli KTE10]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jinek M]] | ||
| + | [[Category: Loeff L]] | ||
Current revision
Escherichia coli SduA complex
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