1lua

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[[Image:1lua.jpg|left|200px]]
 
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==Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP==
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The line below this paragraph, containing "STRUCTURE_1lua", creates the "Structure Box" on the page.
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<StructureSection load='1lua' size='340' side='right'caption='[[1lua]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lua]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens_AM1 Methylorubrum extorquens AM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LUA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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{{STRUCTURE_1lua| PDB=1lua | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lua OCA], [https://pdbe.org/1lua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lua RCSB], [https://www.ebi.ac.uk/pdbsum/1lua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lua ProSAT]</span></td></tr>
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</table>
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'''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP'''
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== Function ==
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[https://www.uniprot.org/uniprot/MTDA_METEA MTDA_METEA] Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lu/1lua_consurf.spt"</scriptWhenChecked>
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1LUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUA OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176390 12176390]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lua ConSurf].
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[[Category: Methylobacterium extorquens]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Demmer, U.]]
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</StructureSection>
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[[Category: Ermler, U.]]
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[[Category: Large Structures]]
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[[Category: Grabarse, W.]]
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[[Category: Methylorubrum extorquens AM1]]
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[[Category: Hagemeier, C H.]]
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[[Category: Demmer U]]
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[[Category: Roth, A.]]
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[[Category: Ermler U]]
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[[Category: Vorholt, J A.]]
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[[Category: Grabarse W]]
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[[Category: Warkentin, E.]]
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[[Category: Hagemeier CH]]
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[[Category: Alpha/beta twisted open sheet structure]]
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[[Category: Roth A]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:17:44 2008''
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[[Category: Vorholt JA]]
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[[Category: Warkentin E]]

Current revision

Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP

PDB ID 1lua

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