1lzl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:37, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1lzl.gif|left|200px]]
 
-
<!--
+
==Bacterial Heroin Esterase==
-
The line below this paragraph, containing "STRUCTURE_1lzl", creates the "Structure Box" on the page.
+
<StructureSection load='1lzl' size='340' side='right'caption='[[1lzl]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1lzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LZL FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lzl OCA], [https://pdbe.org/1lzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lzl RCSB], [https://www.ebi.ac.uk/pdbsum/1lzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lzl ProSAT]</span></td></tr>
-
{{STRUCTURE_1lzl| PDB=1lzl | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''Bacterial Heroin Esterase'''
+
[https://www.uniprot.org/uniprot/O06441_RHOSO O06441_RHOSO]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
The crystal structures of an acetyl esterase, HerE, and its complex with an inhibitor dimethylarsinic acid have been determined at 1.30- and 1.45-A resolution, respectively. Although the natural substrate for the enzyme is unknown, HerE hydrolyzes the acetyl groups from heroin to yield morphine and from phenyl acetate to yield phenol. Recently, the activity of the enzyme toward heroin has been exploited to develop a heroin biosensor, which affords higher sensitivity than other currently available detection methods. The crystal structure reveals a single domain with the canonical alpha/beta hydrolase fold with an acyl binding pocket that snugly accommodates the acetyl substituent of the substrate and three backbone amides that form a tripartite oxyanion hole. In addition, a covalent adduct was observed between the active site serine and dimethylarsinic acid, which inhibits the enzyme. This crystal structure provides the first example of an As-containing compound in a serine esterase active site and the first example of covalent modification of serine by arsenic. Thus, the HerE complex reveals the structural basis for the broad scope inhibition of serine hydrolases by As(V)-containing organic compounds.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/1lzl_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
1LZL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZL OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lzl ConSurf].
-
Observation of an arsenic adduct in an acetyl esterase crystal structure., Zhu X, Larsen NA, Basran A, Bruce NC, Wilson IA, J Biol Chem. 2003 Jan 17;278(3):2008-14. Epub 2002 Nov 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12421810 12421810]
+
<div style="clear:both"></div>
-
[[Category: Rhodococcus sp.]]
+
__TOC__
-
[[Category: Single protein]]
+
</StructureSection>
-
[[Category: Basran, A.]]
+
[[Category: Large Structures]]
-
[[Category: Bruce, N C.]]
+
[[Category: Rhodococcus sp]]
-
[[Category: Larsen, N A.]]
+
[[Category: Basran A]]
-
[[Category: Wilson, I A.]]
+
[[Category: Bruce NC]]
-
[[Category: Zhu, X.]]
+
[[Category: Larsen NA]]
-
[[Category: Alpha/beta hydrolase]]
+
[[Category: Wilson IA]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:27:20 2008''
+
[[Category: Zhu X]]

Current revision

Bacterial Heroin Esterase

PDB ID 1lzl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lzl"
Personal tools