1lxi

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(New page: 200px<br /> <applet load="1lxi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lxi, resolution 2.00&Aring;" /> '''Refinement of BMP7 ...)
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[[Image:1lxi.gif|left|200px]]<br />
 
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<applet load="1lxi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lxi, resolution 2.00&Aring;" />
 
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'''Refinement of BMP7 crystal structure'''<br />
 
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==Overview==
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==Refinement of BMP7 crystal structure==
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Activins and bone morphogenetic proteins (BMPs) elicit diverse biological, responses by signaling through two pairs of structurally related type I, and type II receptors. Here we report the crystal structure of BMP7 in, complex with the extracellular domain (ECD) of the activin type II, receptor. Our structure produces a compelling four-receptor model, revealing that the types I and II receptor ECDs make no direct contacts., Nevertheless, we find that truncated receptors lacking their cytoplasmic, domain retain the ability to cooperatively assemble in the cell membrane., Also, the affinity of BMP7 for its low-affinity type I receptor ECD, increases 5-fold in the presence of its type II receptor ECD. Taken, together, our results provide a view of the ligand-mediated cooperative, assembly of BMP and activin receptors that does not rely on, receptor-receptor contacts.
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<StructureSection load='1lxi' size='340' side='right'caption='[[1lxi]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lxi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LXI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lxi OCA], [https://pdbe.org/1lxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lxi RCSB], [https://www.ebi.ac.uk/pdbsum/1lxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lxi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BMP7_HUMAN BMP7_HUMAN] Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lx/1lxi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lxi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Activins and bone morphogenetic proteins (BMPs) elicit diverse biological responses by signaling through two pairs of structurally related type I and type II receptors. Here we report the crystal structure of BMP7 in complex with the extracellular domain (ECD) of the activin type II receptor. Our structure produces a compelling four-receptor model, revealing that the types I and II receptor ECDs make no direct contacts. Nevertheless, we find that truncated receptors lacking their cytoplasmic domain retain the ability to cooperatively assemble in the cell membrane. Also, the affinity of BMP7 for its low-affinity type I receptor ECD increases 5-fold in the presence of its type II receptor ECD. Taken together, our results provide a view of the ligand-mediated cooperative assembly of BMP and activin receptors that does not rely on receptor-receptor contacts.
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==About this Structure==
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The BMP7/ActRII extracellular domain complex provides new insights into the cooperative nature of receptor assembly.,Greenwald J, Groppe J, Gray P, Wiater E, Kwiatkowski W, Vale W, Choe S Mol Cell. 2003 Mar;11(3):605-17. PMID:12667445<ref>PMID:12667445</ref>
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1LXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LXI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The BMP7/ActRII extracellular domain complex provides new insights into the cooperative nature of receptor assembly., Greenwald J, Groppe J, Gray P, Wiater E, Kwiatkowski W, Vale W, Choe S, Mol Cell. 2003 Mar;11(3):605-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12667445 12667445]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1lxi" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Choe, S.]]
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[[Category: Greenwald, J.]]
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[[Category: Groppe, J.]]
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[[Category: Kwiatkowski, W.]]
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[[Category: NAG]]
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[[Category: cystine-knot growth factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:04:57 2007''
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==See Also==
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*[[Bone morphogenetic protein 3D structures|Bone morphogenetic protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Choe S]]
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[[Category: Greenwald J]]
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[[Category: Groppe J]]
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[[Category: Kwiatkowski W]]

Current revision

Refinement of BMP7 crystal structure

PDB ID 1lxi

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