8smj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Chimeric ETS-domain of murine PU.1 harboring the corresponding beta-strand 3 (S3) residues from murine Ets-1 in complex with d(AATAAGCGGAATGGGG)== | |
| + | <StructureSection load='8smj' size='340' side='right'caption='[[8smj]], [[Resolution|resolution]] 1.39Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8smj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SMJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8smj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8smj OCA], [https://pdbe.org/8smj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8smj RCSB], [https://www.ebi.ac.uk/pdbsum/8smj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8smj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [https://www.uniprot.org/uniprot/SPI1_MOUSE SPI1_MOUSE] Note=Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion. | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SPI1_MOUSE SPI1_MOUSE] Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.<ref>PMID:8626664</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleobases such as inosine have been extensively utilized to map direct contacts by proteins in the DNA groove. Their deployment as targeted probes of dynamics and hydration, which are dominant thermodynamic drivers of affinity and specificity, has been limited by a paucity of suitable experimental models. We report a joint crystallographic, thermodynamic, and computational study of the bidentate complex of the arginine side chain with a Watson-Crick guanine (ArgxGC), a highly specific configuration adopted by major transcription factors throughout the eukaryotic branches in the Tree of Life. Using the ETS-family factor PU.1 as a high-resolution structural framework, inosine substitution for guanine resulted in a sharp dissection of conformational dynamics and hydration and elucidated their role in the DNA specificity of PU.1. Our work suggests an under-exploited utility of modified nucleobases in untangling the structural thermodynamics of interactions, such as the ArgxGC motif, where direct and indirect readout are tightly integrated. | ||
| - | + | Dissection of integrated readout reveals the structural thermodynamics of DNA selection by transcription factors.,Vernon TN, Terrell JR, Albrecht AV, Germann MW, Wilson WD, Poon GMK Structure. 2024 Jan 4;32(1):83-96.e4. doi: 10.1016/j.str.2023.11.003. Epub 2023 , Dec 1. PMID:38042148<ref>PMID:38042148</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8smj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Poon GMK]] | ||
| + | [[Category: Terrell JR]] | ||
Current revision
Chimeric ETS-domain of murine PU.1 harboring the corresponding beta-strand 3 (S3) residues from murine Ets-1 in complex with d(AATAAGCGGAATGGGG)
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