8tru
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a CE15 from Fibrobacter succinogenes subsp. succinogenes S85== | |
| + | <StructureSection load='8tru' size='340' side='right'caption='[[8tru]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8tru]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fibrobacter_succinogenes_subsp._succinogenes_S85 Fibrobacter succinogenes subsp. succinogenes S85]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TRU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tru OCA], [https://pdbe.org/8tru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tru RCSB], [https://www.ebi.ac.uk/pdbsum/8tru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tru ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C9RKY6_FIBSS C9RKY6_FIBSS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glucuronoyl esterases (GEs) are carbohydrate active enzymes in carbohydrate esterase family 15 which are involved in the hydrolysis of lignin-carbohydrate complexes. They are encoded by a wide range of aerobic and anaerobic fungi and bacteria inhabiting diverse environments. The rumen microbiome is a complex microbial community with a wide array of enzymes that specialize in deconstructing plant cell wall carbohydrates. Enzymes from the rumen tend to show low similarity to homologues found in other environments, making the rumen microbiome a promising source for the discovery of novel enzymes. Using a combination of phylogenetic and structural analysis, we investigated the structure-function relationship of GEs from the rumen bacteria Fibrobacter succinogenes and Ruminococcus flavefaciens, and from the rumen fungus, Piromyces rhizinflata. All adopt a canonical alpha/beta hydrolase fold and possess a structurally conserved Ser-His-Glu/Asp catalytic triad. Structural variations in the enzymes are localized to loops surrounding the active site. Analysis of the active site structures in these enzymes emphasized the importance of structural plasticity in GEs with non-canonical active site conformations. We hypothesize that interkingdom HGT events may have contributed to the diversity of GEs in the rumen, and this is demonstrated by the phylogenetic and structural similarity observed between rumen bacterial and fungal GEs. This study advances our understanding of the structure-function relationship in glucuronoyl esterases and illuminates the evolutionary dynamics that contribute to enzyme diversity in the rumen microbiome. | ||
| - | + | Structural, Biochemical, and Phylogenetic Analysis of Bacterial and Fungal Carbohydrate Esterase Family 15 Glucuronoyl Esterases in the Rumen.,Gruninger RJ, Kevorkova M, Low KE, Jones DR, Worrall L, McAllister TA, Abbott DW Protein J. 2024 Aug;43(4):910-922. doi: 10.1007/s10930-024-10221-0. Epub 2024 Aug , 17. PMID:39153129<ref>PMID:39153129</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8tru" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Fibrobacter succinogenes subsp. succinogenes S85]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gruninger RJ]] | ||
| + | [[Category: Jones DR]] | ||
Current revision
Crystal structure of a CE15 from Fibrobacter succinogenes subsp. succinogenes S85
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