1m82

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[[Image:1m82.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF THE COMPLEMENTARY RNA PROMOTER OF INFLUENZA A VIRUS==
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The line below this paragraph, containing "STRUCTURE_1m82", creates the "Structure Box" on the page.
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<StructureSection load='1m82' size='340' side='right'caption='[[1m82]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1m82]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M82 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m82 OCA], [https://pdbe.org/1m82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m82 RCSB], [https://www.ebi.ac.uk/pdbsum/1m82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m82 ProSAT]</span></td></tr>
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{{STRUCTURE_1m82| PDB=1m82 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''SOLUTION STRUCTURE OF THE COMPLEMENTARY RNA PROMOTER OF INFLUENZA A VIRUS'''
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== Publication Abstract from PubMed ==
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==Overview==
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Influenza A virus replication requires the interaction of viral RNA-dependent RNA polymerase (RdRp) with promoters in both the RNA genome (vRNA) and the full-length complementary RNA (cRNA) which serve as templates for the generation of new vRNAs. Although RdRp binds both promoters effectively, it must also discriminate between them because they serve different functional roles in the viral life cycle. Even though the inherent asymmetry between two RNA promoters is considered as a cause of the differential recognition by the RdRp, the structural basis for the ability of the RdRp to recognize the RNA promoters and discriminate effectively between them remains unsolved. Here we report the structure of the cRNA promoter of influenza A virus as determined by heteronuclear magnetic resonance spectroscopy. The terminal region is extremely unstable and does not have a rigid structure. The major groove of the internal loop is widened by the displacement of a novel A*(UU) motif toward the minor groove. These internal loop residues show distinguishable dynamic characters, with differing motional timescales for each residue. Comparison of the cRNA promoter structure with that of the vRNA promoter reveals common structural and dynamic elements in the internal loop, but also differences that provide insight into how the viral RdRp differentially recognizes the cRNA and vRNA promoters.
Influenza A virus replication requires the interaction of viral RNA-dependent RNA polymerase (RdRp) with promoters in both the RNA genome (vRNA) and the full-length complementary RNA (cRNA) which serve as templates for the generation of new vRNAs. Although RdRp binds both promoters effectively, it must also discriminate between them because they serve different functional roles in the viral life cycle. Even though the inherent asymmetry between two RNA promoters is considered as a cause of the differential recognition by the RdRp, the structural basis for the ability of the RdRp to recognize the RNA promoters and discriminate effectively between them remains unsolved. Here we report the structure of the cRNA promoter of influenza A virus as determined by heteronuclear magnetic resonance spectroscopy. The terminal region is extremely unstable and does not have a rigid structure. The major groove of the internal loop is widened by the displacement of a novel A*(UU) motif toward the minor groove. These internal loop residues show distinguishable dynamic characters, with differing motional timescales for each residue. Comparison of the cRNA promoter structure with that of the vRNA promoter reveals common structural and dynamic elements in the internal loop, but also differences that provide insight into how the viral RdRp differentially recognizes the cRNA and vRNA promoters.
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==About this Structure==
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Solution structure of the influenza A virus cRNA promoter: implications for differential recognition of viral promoter structures by RNA-dependent RNA polymerase.,Park CJ, Bae SH, Lee MK, Varani G, Choi BS Nucleic Acids Res. 2003 Jun 1;31(11):2824-32. PMID:12771209<ref>PMID:12771209</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M82 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the influenza A virus cRNA promoter: implications for differential recognition of viral promoter structures by RNA-dependent RNA polymerase., Park CJ, Bae SH, Lee MK, Varani G, Choi BS, Nucleic Acids Res. 2003 Jun 1;31(11):2824-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12771209 12771209]
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</div>
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[[Category: Bae, S H.]]
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<div class="pdbe-citations 1m82" style="background-color:#fffaf0;"></div>
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[[Category: Choi, B S.]]
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== References ==
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[[Category: Lee, M K.]]
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<references/>
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[[Category: Park, C J.]]
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__TOC__
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[[Category: Varani, G.]]
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</StructureSection>
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[[Category: A-form helix]]
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[[Category: Large Structures]]
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[[Category: Asymmetric internal loop]]
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[[Category: Bae S-H]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:45:04 2008''
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[[Category: Choi B-S]]
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[[Category: Lee M-K]]
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[[Category: Park C-J]]
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[[Category: Varani G]]

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SOLUTION STRUCTURE OF THE COMPLEMENTARY RNA PROMOTER OF INFLUENZA A VIRUS

PDB ID 1m82

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