1m8x

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(New page: 200px<br /> <applet load="1m8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m8x, resolution 2.20&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1m8x.gif|left|200px]]<br />
 
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<applet load="1m8x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m8x, resolution 2.20&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA==
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Puf proteins are developmental regulators that control mRNA stability and, translation by binding sequences in the 3' untranslated regions of their, target mRNAs. We have determined the structure of the RNA binding domain, of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand., The RNA binds the concave surface of the molecule, where each of the, protein's eight repeats makes contacts with a different RNA base via three, amino acid side chains at conserved positions. We have mutated these three, side chains in one repeat, thereby altering the sequence specificity of, Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is, achieved using multiple copies of a simple repeated motif.
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<StructureSection load='1m8x' size='340' side='right'caption='[[1m8x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m8x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8X FirstGlance]. <br>
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1M8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M8X OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8x OCA], [https://pdbe.org/1m8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8x RCSB], [https://www.ebi.ac.uk/pdbsum/1m8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8x ProSAT]</span></td></tr>
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==Reference==
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</table>
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Modular recognition of RNA by a human pumilio-homology domain., Wang X, McLachlan J, Zamore PD, Hall TM, Cell. 2002 Aug 23;110(4):501-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12202039 12202039]
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== Function ==
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[https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8x ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hall, T.M.T.]]
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[[Category: Hall TMT]]
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[[Category: McLachlan, J.]]
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[[Category: McLachlan J]]
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[[Category: Wang, X.]]
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[[Category: Wang X]]
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[[Category: Zamore, P.D.]]
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[[Category: Zamore PD]]
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[[Category: nanos response element]]
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[[Category: puf domain]]
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[[Category: pumilio-homology domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:08:51 2007''
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Current revision

CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA

PDB ID 1m8x

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