We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1m9e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:41, 14 February 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1m9e.gif|left|200px]]<br />
 
-
<applet load="1m9e" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1m9e, resolution 1.72&Aring;" />
 
-
'''X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type H87A Complex.'''<br />
 
-
==Overview==
+
==X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type H87A Complex.==
-
Cyclophilins constitute a ubiquitous protein family whose functions, include protein folding, transport and signaling. They possess both, sequence-specific binding and proline cis-trans isomerase activities, as, exemplified by the interaction between cyclophilin A (CypA) and the HIV-1, CA protein. Here, we report crystal structures of CypA in complex with, HIV-1 CA protein variants that bind preferentially with the substrate, proline residue in either the cis or the trans conformation. Cis- and, trans-Pro substrates are accommodated within the enzyme active site by, rearrangement of their N-terminal residues and with minimal distortions in, the path of the main chain. CypA Arg55 guanidinium group probably, facilitates catalysis by anchoring the substrate proline oxygen and, stabilizing sp3 hybridization of the proline nitrogen in the transition, state.
+
<StructureSection load='1m9e' size='340' side='right'caption='[[1m9e]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1m9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9E FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9e OCA], [https://pdbe.org/1m9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9e RCSB], [https://www.ebi.ac.uk/pdbsum/1m9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9e ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PPIA_HUMAN PPIA_HUMAN] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m9e_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m9e ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1M9E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M9E OCA].
+
*[[Cyclophilin 3D structures|Cyclophilin 3D structures]]
-
 
+
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-
==Reference==
+
__TOC__
-
Structural insights into the catalytic mechanism of cyclophilin A., Howard BR, Vajdos FF, Li S, Sundquist WI, Hill CP, Nat Struct Biol. 2003 Jun;10(6):475-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12730686 12730686]
+
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Human immunodeficiency virus 1]]
-
[[Category: Peptidylprolyl isomerase]]
+
[[Category: Large Structures]]
-
[[Category: Protein complex]]
+
[[Category: Hill CP]]
-
[[Category: Hill, C.P.]]
+
[[Category: Howard BR]]
-
[[Category: Howard, B.R.]]
+
[[Category: Li S]]
-
[[Category: Li, S.]]
+
[[Category: Sundquist WI]]
-
[[Category: Sundquist, W.I.]]
+
[[Category: Vajdos FF]]
-
[[Category: Vajdos, F.F.]]
+
-
[[Category: capsid]]
+
-
[[Category: cyclophilin a]]
+
-
[[Category: hiv-1]]
+
-
[[Category: isomerase]]
+
-
[[Category: rotamase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:09:04 2007''
+

Current revision

X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type H87A Complex.

PDB ID 1m9e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m9e"
Personal tools