1m9s

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[[Image:1m9s.jpg|left|200px]]
 
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==Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.==
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The line below this paragraph, containing "STRUCTURE_1m9s", creates the "Structure Box" on the page.
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<StructureSection load='1m9s' size='340' side='right'caption='[[1m9s]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1m9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
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{{STRUCTURE_1m9s| PDB=1m9s | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9s OCA], [https://pdbe.org/1m9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9s RCSB], [https://www.ebi.ac.uk/pdbsum/1m9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9s ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.'''
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== Function ==
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[https://www.uniprot.org/uniprot/INLB_LISMO INLB_LISMO] Mediates the entry of Listeria monocytogenes into cells.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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InlB, a surface-localized protein of Listeria monocytogenes, induces phagocytosis in non-phagocytic mammalian cells by activating Met, a receptor tyrosine kinase. InlB also binds glycosaminoglycans and the protein gC1q-R, two additional host ligands implicated in invasion. We present the structure of InlB, revealing a highly elongated molecule with leucine-rich repeats that bind Met at one end, and GW domains that dissociably bind the bacterial surface at the other. Surprisingly, the GW domains are seen to resemble SH3 domains. Despite this, GW domains are unlikely to act as functional mimics of SH3 domains since their potential proline-binding sites are blocked or destroyed. However, we do show that the GW domains, in addition to binding glycosaminoglycans, bind gC1q-R specifically, and that this binding requires release of InlB from the bacterial surface. Dissociable attachment to the bacterial surface via the GW domains may be responsible for restricting Met activation to a small, localized area of the host cell and for coupling InlB-induced host membrane dynamics with bacterial proximity during invasion.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m9s_consurf.spt"</scriptWhenChecked>
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1M9S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9S OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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GW domains of the Listeria monocytogenes invasion protein InlB are SH3-like and mediate binding to host ligands., Marino M, Banerjee M, Jonquieres R, Cossart P, Ghosh P, EMBO J. 2002 Nov 1;21(21):5623-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12411480 12411480]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m9s ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Listeria monocytogenes]]
[[Category: Listeria monocytogenes]]
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[[Category: Single protein]]
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[[Category: Banerjee M]]
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[[Category: Banerjee, M.]]
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[[Category: Cossart P]]
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[[Category: Cossart, P.]]
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[[Category: Ghosh P]]
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[[Category: Ghosh, P.]]
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[[Category: Jonquieres R]]
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[[Category: Jonquieres, R.]]
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[[Category: Marino M]]
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[[Category: Marino, M.]]
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[[Category: Cell invasion]]
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[[Category: Gw domain]]
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[[Category: Internalin]]
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[[Category: Sh3 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:48:12 2008''
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Current revision

Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.

PDB ID 1m9s

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