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1mgs

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(New page: 200px<br /> <applet load="1mgs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mgs" /> '''THE SOLUTION STRUCTURE OF MELANOMA GROWTH S...)
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[[Image:1mgs.gif|left|200px]]<br />
 
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<applet load="1mgs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mgs" />
 
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'''THE SOLUTION STRUCTURE OF MELANOMA GROWTH STIMULATING ACTIVITY'''<br />
 
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==Overview==
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==THE SOLUTION STRUCTURE OF MELANOMA GROWTH STIMULATING ACTIVITY==
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The solution structure of melanoma growth stimulating activity (MGSA), a, dimeric chemokine consisting of 73 residues per monomer, has been, determined using two-dimensional homonuclear and three-dimensional, heteronuclear NMR spectroscopy. Structure calculations were carried out, using a hybrid distance geometry-simulated annealing approach with the, programs DGII and X-PLOR. The structure is based on a total of 2362, experimental restraints, comprising 2150 NOE-derived distance restraints, (2076 unambiguous intrasubunit restraints, 60 unambiguous intersubunit, restraints, and 14 ambiguous restraints with potential contributions from, both intra- and intersubunit NOEs), 84 distance restraints for 42 backbone, hydrogen bonds, and 128 torsion angle restraints. The ambiguous distance, restraints were treated using a target function which accounts for both, intra- and intermolecular contributions to the NOE intensity. A total of, 25 structures were calculated, with the backbone (N, C alpha, C) atomic, r.m.s. distribution about the mean coordinates for residues 8 to 69 being, 0.44(+/- 0.10) A for the dimer and 0.34(+/- 0.07) A for the individual, monomers. The N- and C-terminal residues (1 to 7 and 70 to 73, respectively) are disordered. The overall structure of the MGSA dimer is, similar to that reported previously for the NMR and X-ray structures of, interleukin-8 (IL-8), and consists of a six-stranded antiparallel, beta-sheet packed against two C-terminal antiparallel alpha-helices. A, best fit superposition of the NMR structure of MGSA on the X-ray and NMR, structures of IL-8 yields backbone atomic r.m.s. differences of 0.99 and, 1.28 A, respectively for individual monomers, and 1.08 and 1.82 A, respectively for the dimers (using MGSA residues 8 to 14 and 19 to 69). In, general, the MGSA structure resembles the IL-8 X-ray structure more than, it does the IL-8 NMR structure. At the tertiary (monomer) level the two, main differences between the MGSA solution structure and IL-8 NMR, structure involve the loops between residues 14 to 19 and between residues, 30 to 38. At the quaternary (dimer) level the difference results from, differing angles between the beta-strands which form the dimer interface, and is manifest as a different interhelical separation (distance of, closest approach between the two helices is 15.3 A in the IL-8 NMR, structure and 11.7 (+/- 0.4) A in the MGSA structure).
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<StructureSection load='1mgs' size='340' side='right'caption='[[1mgs]]' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1mgs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MGS FirstGlance]. <br>
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Known diseases associated with this structure: AIDS, resistance to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600835 600835]], Osteogenesis imperfecta, type VIII OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=610339 610339]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mgs OCA], [https://pdbe.org/1mgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mgs RCSB], [https://www.ebi.ac.uk/pdbsum/1mgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mgs ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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1MGS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MGS OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/GROA_HUMAN GROA_HUMAN] Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.<ref>PMID:10095777</ref> <ref>PMID:2670560</ref>
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==Reference==
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== Evolutionary Conservation ==
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The solution structure of melanoma growth stimulating activity., Fairbrother WJ, Reilly D, Colby TJ, Hesselgesser J, Horuk R, J Mol Biol. 1994 Sep 23;242(3):252-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8089846 8089846]
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mgs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mgs ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fairbrother, W.J.]]
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[[Category: Fairbrother WJ]]
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[[Category: chemokine(growth factor)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:11:00 2007''
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THE SOLUTION STRUCTURE OF MELANOMA GROWTH STIMULATING ACTIVITY

PDB ID 1mgs

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